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Sensors (Basel, Switzerland)
Jul 29, 2019;19 (15):

Enhanced Binding Efficiency of Microcantilever Biosensor for the Detection of Yersinia.

Xiaochen Liu, Lihao Wang, Junyuan Zhao, Yinfang Zhu, Jinling Yang, Fuhua Yang
Author Information
  1. Xiaochen Liu: Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.
  2. Lihao Wang: Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.
  3. Junyuan Zhao: Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.
  4. Yinfang Zhu: Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China. yfzhu@semi.ac.cn.
  5. Jinling Yang: Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China. jlyang@semi.ac.cn.
  6. Fuhua Yang: Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.
PMID: 31362399 DOI: 10.3390/s19153326

Abstract

A novel microcantilever sensor was batch fabricated for Yersinia detection. The microcantilever surface modification method was optimized by introducing a secondary antibody to increase the number of binding sites. A novel microfluidic platform was designed and fabricated successfully. A 30 μL solution could fully react with the microcantilever surface. Those routines enhanced the binding efficiency between the target and receptor on the microcantilever. With this novel designed microfluidic platform, the specific adsorption of 10 Yersinia on the beam surface with modified F1 antibody was significantly enhanced.

Keywords

Yersinia binding efficiency microcantilever microfluidic system

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MeSH Term

Antibodies
Binding Sites
Biosensing Techniques
Humans
Microfluidics
Surface Properties
Yersinia
Yersinia Infections

Chemicals

Antibodies
Journal Article

Word Cloud

Created with Highcharts 10.0.0microcantileverYersinianovelsurfacebindingmicrofluidicfabricatedantibodyplatformdesignedenhancedefficiencysensorbatchdetectionmodificationmethodoptimizedintroducingsecondaryincreasenumbersitessuccessfully30μLsolutionfullyreactroutinestargetreceptorspecificadsorption10beammodifiedF1significantlyEnhancedBindingEfficiencyMicrocantileverBiosensorDetectionsystem

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