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Scientific reports
08 02, 2019;9 (1): 11253.

Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis.

Xiaomei Liu, Jingchun Tang, Lan Wang, Rutao Liu
Author Information
  1. Xiaomei Liu: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Research Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, China.
  2. Jingchun Tang: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Research Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, China. tangjch@nankai.edu.cn.
  3. Lan Wang: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Research Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, China.
  4. Rutao Liu: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, 72# Jimo Binhai Road, Qingdao, Shandong, 266237, P.R. China.
PMID: 31375702 DOI: 10.1038/s41598-019-46833-1

Abstract

In this research, antibiotic-producing bacteria, Streptomyces coelicolor (S. coelicolor) M145, was exposed to copper oxide (CuO) particles to investigate the effects of nano-particles (NPs) on antibiotic production. Results showed that a higher yield of antibiotics was obtained with smaller particle sizes of CuO NPs. When exposed to 10 mg/L of 40 nm CuO NPs, the maximum amount of actinorhodin (ACT) obtained was 2.6 mg/L after 144 h, which was 2.0-fold greater than that of control. However, the process was inhibited when the concentration of CuO NPs was increased to higher than 20 mg/L. Transcriptome analysis showed that all the genes involved in the ACT cluster were significantly up-regulated after exposure to 10 mg/L NPs, which could be the direct cause of the increase of ACT production. Additionally, some genes related to the generation of acetyl-coA were up-regulated. In this way, CuO NPs led to an increase of secondary metabolites. The mechanism related to these changes indicated that nano-particle‒induced ROS and Cu played synergetic roles in promoting ACT biosynthesis. This is a first report suggesting that CuO NPs had a significant effect on antibiotic production, which will be helpful in understanding the mechanism of antibiotic production in nature.

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MeSH Term

Anthraquinones
Anti-Bacterial Agents
Biosynthetic Pathways
Copper
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Nanoparticles
Reactive Oxygen Species
Streptomyces coelicolor

Chemicals

Anthraquinones
Anti-Bacterial Agents
Reactive Oxygen Species
Copper
actinorhodin
cupric oxide
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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