Antibacterial Silver-Conjugated Magnetic Nanoparticles: Design, Synthesis and Bactericidal Effect.
Anastasiia B Shatan, Kristýna Venclíková, Beata A Zasońska, Vitalii Patsula, Ognen Pop-Georgievski, Eduard Petrovský, Daniel Horák
Author Information
Anastasiia B Shatan: Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic.
Kristýna Venclíková: Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic.
Beata A Zasońska: Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic.
Vitalii Patsula: Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic.
Ognen Pop-Georgievski: Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic.
Eduard Petrovský: Institute of Geophysics, Czech Academy of Sciences, Boční II/1401, 141 31, Prague 4, Czech Republic.
Daniel Horák: Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06, Prague 6, Czech Republic. horak@imc.cas.cz. ORCID
PURPOSE: The aim was to design and thoroughly characterize monodisperse FeO@SiO-Ag nanoparticles with strong antibacterial properties, which makes them a candidate for targeting bacterial infections. METHODS: The monodisperse FeO nanoparticles were prepared by oleic acid-stabilized thermal decomposition of Fe(III) oleate; the particles were coated with silica shell using a water-in-oil reverse microemulsion, involving hydrolysis and condensation of tetramethyl orthosilicate. Resulting FeO@SiO particles were modified by (3-mercaptopropyl)trimethoxysilane to introduce 1.1 mmol SH/g. Finally, the FeO@SiO-SH nanoparticles were decorated with silver nanoclusters formed by reduction of silver nitrate with NaBH. The particles were analyzed by FTIR, X-ray photoelectron and atomic absorption spectroscopy, dynamic light scattering and vibrating sample magnetometry. The antibacterial activity of the FeO@SiO and FeO@SiO-Ag nanoparticles was tested against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria cultivated on Luria agar plates or in Luria broth. RESULTS: The superparamagnetic FeO@SiO-Ag nanoparticles (21 nm in diameter; saturation magnetization 26 A∙m/kg) were successfully obtained and characterized. Inhibitory and toxic effects against bacteria were documented by incubation of the FeO@SiO-Ag nanoparticles with Staphylococcus aureus and Escherichia coli. CONCLUSIONS: The combination of magnetic properties together with bactericidal effects is suitable for the disinfection of medical instruments, water purification, food packaging, etc.
