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RNA biology
12, 2019;16 (12): 1697-1710.

Synonymous SNPs of viral genes facilitate virus to escape host antiviral RNAi immunity.

Yuechao Sun, Yu Zhang, Xiaobo Zhang
Author Information
  1. Yuechao Sun: College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People's Republic of China.
  2. Yu Zhang: College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People's Republic of China.
  3. Xiaobo Zhang: College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou, People's Republic of China.
PMID: 31416386 DOI: 10.1080/15476286.2019.1656026

Abstract

Synonymous single nucleotide polymorphisms (SNPs) are involved in codon usage preference or mRNA splicing. Up to date, however, the role of synonymous SNPs in immunity remains unclear. To address this issue, the SNPs of white spot syndrome virus (WSSV) were characterized in shrimp in the present study. Our results indicated that there existed synonymous SNPs in the mRNAs of wsv151 and wsv226, two viral genes of WSSV. In the presence of SNP siRNA, wild-type siRNA, wild-type mRNA and SNP mRNA of wsv151 or wsv226, RNAi was significantly suppressed, showing that the synonymous SNPs of wsv151 and wsv226 played negative roles in host siRNA pathway due to mismatch of siRNA with its target. In insect cells, the mismatch, caused by synonymous SNPs of wsv151 or wsv226, between siRNA and its target inhibited the host RNAi. Furthermore, the data revealed that the co-injection of SNP siRNA and wild-type siRNA of wsv151 or wsv226 into WSSV-infected shrimp led to a significant increase of WSSV copies compared with that of SNP siRNA alone or wild-type siRNA alone, indicating that the synonymous SNPs of viral genes could be a strategy of virus escaping host siRNA pathway in shrimp . Therefore, our study provided novel insights into the underlying mechanism of virus escaping host antiviral RNAi immunity by synonymous SNPs of viral genes.

Keywords

Synonymous single nucleotide polymorphism WSSV shrimp siRNA

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MeSH Term

Animals
Arthropod Proteins
Base Pairing
Base Sequence
DEAD-box RNA Helicases
Immune Evasion
Penaeidae
Polymorphism, Single Nucleotide
RNA Interference
RNA Splicing
RNA, Messenger
RNA, Small Interfering
RNA, Viral
Viral Load
White spot syndrome virus 1

Chemicals

Arthropod Proteins
RNA, Messenger
RNA, Small Interfering
RNA, Viral
DEAD-box RNA Helicases
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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