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Nature methods
09, 2019;16 (9): 830-842.

Biological imaging of chemical bonds by stimulated Raman scattering microscopy.

Fanghao Hu, Lixue Shi, Wei Min
Author Information
  1. Fanghao Hu: Department of Chemistry, Columbia University, New York, NY, USA.
  2. Lixue Shi: Department of Chemistry, Columbia University, New York, NY, USA.
  3. Wei Min: Department of Chemistry, Columbia University, New York, NY, USA. wm2256@columbia.edu.
PMID: 31471618 DOI: 10.1038/s41592-019-0538-0

Abstract

All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.

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MeSH Term

Animals
Humans
Macromolecular Substances
Molecular Imaging
Nonlinear Optical Microscopy

Chemicals

Macromolecular Substances
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review
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