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Genes & genomics
12, 2019;41 (12): 1383-1396.

A transcriptome analysis uncovers Panax notoginseng resistance to Fusarium solani induced by methyl jasmonate.

Diqiu Liu, Qin Zhao, Xiuming Cui, Rui Chen, Xin Li, Bingling Qiu, Feng Ge
Author Information
  1. Diqiu Liu: Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China. Diqiuliu@126.com.
  2. Qin Zhao: Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China.
  3. Xiuming Cui: Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China.
  4. Rui Chen: Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China.
  5. Xin Li: Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China.
  6. Bingling Qiu: Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China.
  7. Feng Ge: Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China.
PMID: 31493262 DOI: 10.1007/s13258-019-00865-z

Abstract

BACKGROUND: Panax notoginseng is a famous Chinese herbal medicine, but the root rot disease mainly caused by Fusarium solani severely reduces the yield and quality of its medicinal materials.
OBJECTIVE: The defense priming in P. notoginseng through exogenous application of signaling molecule will supply theoretical support for the exogenous regulation of disease resistance in P. notoginseng.
METHODS: In this study, the exogenous application of methyl jasmonate (MeJA) increased P. notoginseng's resistance to F. solani. Furthermore, the P. notoginseng transcriptome during F. solani infection was investigated through next-generation sequencing to uncover the resistance mechanism of P. notogingseng induced by MeJA.
RESULTS: The de novo assembly of transcriptome sequences produced 80,551 unigenes, and 36,771 of these unigenes were annotated by at least one database. A differentially expressed gene analysis revealed that a large number of genes related to terpenoid backbone biosynthesis, phenylalanine metabolism, and plant-pathogen interactions were predominantly up-regulated by MeJA. Moreover, jasmonic acid (JA) biosynthesis-related genes and the JA signaling pathway genes, such as linoleate 13S-lipoxygenase, allene oxide cyclase, allene oxide synthase, TIFY, defensin, and pathogenesis-related proteins, showed increased transcriptional levels after inoculation with F. solani. Notably, according to the gene expression analysis, JA and ethylene signaling pathways may act synergistically to positively regulate the defense responses of P. notoginseng to F. solani.
CONCLUSION: JA signaling appears to play a vital role in P. notoginseng responses to F. solani infection, which will be helpful in improving the disease resistance of P. notoginseng cultivars as well as in developing an environmentally friendly biological control method for root rot disease.

Keywords

Differentially expressed genes Fusarium solani Methyl jasmonate Panax notoginseng Transcriptome

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MeSH Term

Acetates
Cyclopentanes
Disease Resistance
Fusarium
Gene Expression Profiling
Gene Expression Regulation, Plant
Oxylipins
Panax notoginseng
Plant Diseases
RNA-Seq
Transcriptome

Chemicals

Acetates
Cyclopentanes
Oxylipins
methyl jasmonate
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0notoginsengsolaniPresistanceFdiseasesignalinggenesJAPanaxFusariumexogenousjasmonateMeJAtranscriptomeanalysisrootrotdefenseapplicationwillmethylincreasedinfectioninducedunigenesexpressedgenealleneoxideresponsesBACKGROUND:famousChineseherbalmedicinemainlycausedseverelyreducesyieldqualitymedicinalmaterialsOBJECTIVE:primingmoleculesupplytheoreticalsupportregulationMETHODS:studynotoginseng'sFurthermoreinvestigatednext-generationsequencinguncovermechanismnotogingsengRESULTS:denovoassemblysequencesproduced8055136771annotatedleastonedatabasedifferentiallyrevealedlargenumberrelatedterpenoidbackbonebiosynthesisphenylalaninemetabolismplant-pathogeninteractionspredominantlyup-regulatedMoreoverjasmonicacidbiosynthesis-relatedpathwaylinoleate13S-lipoxygenasecyclasesynthaseTIFYdefensinpathogenesis-relatedproteinsshowedtranscriptionallevelsinoculationNotablyaccordingexpressionethylenepathwaysmayactsynergisticallypositivelyregulateCONCLUSION:appearsplayvitalrolehelpfulimprovingcultivarswelldevelopingenvironmentallyfriendlybiologicalcontrolmethoduncoversDifferentiallyMethylTranscriptome

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