Methylsulfonylmethane exhibits bacteriostatic inhibition of Escherichia coli, and Salmonella enterica Kinshasa, in vitro.

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T L Poole, R Benjamin, K J Genovese, D J Nisbet

Author Information

T L Poole: USDA/ARS, Southern Plains Agricultural Research Center, College Station, TX, USA. ORCID
R Benjamin: Bergstrom Nutrition, Vancouver, WA, USA.
K J Genovese: USDA/ARS, Southern Plains Agricultural Research Center, College Station, TX, USA.
D J Nisbet: USDA/ARS, Southern Plains Agricultural Research Center, College Station, TX, USA.

PMID: 31509887 DOI: 10.1111/jam.14446

AIMS: To evaluate antibacterial properties of methylsulfonylmethane (MSM) on Escherichia coli (MDRE21) and Salmonella enterica serovar Kinshasa (SK132).
METHODS AND RESULTS: Bacterial proliferation analysis was measured spectrophotometrically during log phase growth with 0, 3, 5, 7, 10, 12 and 16% MSM. To assess the mechanism of inhibition, cultures were grown overnight with 0-16% MSM and enumerated on unmedicated brain-heart infusion agar (BHIA) or BHIA with 0-16% MSM. The long-term viability studies were done to evaluate the impact of 10% MSM. Absorbance data indicated a dose-dependent inhibition from 0 to 16% MSM. There was no growth of MDRE21 or SK132 on BHIA in 10-16% MSM. Both strains enumerated on unmedicated BHIA from overnight cultures with 10-16% MSM were able to partially recover.
CONCLUSIONS: Recovery after MSM removal may be indicative of a bacteriostatic mechanism of inhibition. The long-term viability studies illustrated that neither MDRE21 nor SK132 could be rescued from 10% MSM after 5 or 6 days respectively.
SIGNIFICANCE AND IMPACT OF THE STUDY: Methylsulfonylmethane antibacterial activity may prove useful during pre or postharvest food safety as a disinfectant. The primary benefit being, its clinical safety to humans. Comparisons to other disinfectants would also need to be done to determine if MSM was superior to those already on the market and would be cost effective.

Salmonella E. coli (all potentially pathogenic types) agriculture antimicrobials disinfection

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Comodity grant $12,000.00/Bergstrom Nutrition
/U.S. Department of Agriculture
/Agricultural Research Service

Anti-Infective Agents

Dimethyl Sulfoxide

Escherichia coli

Food Microbiology

Food Safety

Humans

Microbial Viability

Salmonella enterica

Sulfones

Anti-Infective Agents

Sulfones

dimethyl sulfone

Dimethyl Sulfoxide

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