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Scientific reports
09 18, 2019;9 (1): 13509.

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei.

Luca Peruzza, M S Shekhar, K Vinaya Kumar, A Swathi, K Karthic, Chris Hauton, K K Vijayan
Author Information
  1. Luca Peruzza: School of Ocean and Earth Science, University of Southampton, Hampshire, SO14 3ZH, United Kingdom. luca.peruzza@soton.ac.uk. ORCID
  2. M S Shekhar: Genetics and Biotechnology Unit, Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, R.A. Puram, Chennai, 600004, Tamil Nadu, India.
  3. K Vinaya Kumar: Genetics and Biotechnology Unit, Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, R.A. Puram, Chennai, 600004, Tamil Nadu, India.
  4. A Swathi: Genetics and Biotechnology Unit, Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, R.A. Puram, Chennai, 600004, Tamil Nadu, India.
  5. K Karthic: Genetics and Biotechnology Unit, Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, R.A. Puram, Chennai, 600004, Tamil Nadu, India.
  6. Chris Hauton: School of Ocean and Earth Science, University of Southampton, Hampshire, SO14 3ZH, United Kingdom.
  7. K K Vijayan: Genetics and Biotechnology Unit, Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, R.A. Puram, Chennai, 600004, Tamil Nadu, India.
PMID: 31534145 DOI: 10.1038/s41598-019-49836-0

Abstract

Shrimp aquaculture is severely affected by WSSV. Despite an increasing effort to understand host/virus interaction by characterizing changes in gene expression (GE) following WSSV infection, the majority of published studies have focussed on a single time-point, providing limited insight on the development of host-pathogen interaction over the infection cycle. Using RNA-seq, we contrasted GE in gills of Litopenaeus vannamei at 1.5, 18 and 56 hours-post-infection (hpi), between WSSV-challenged and control shrimps. Time course analysis revealed 5097 differentially expressed genes: 63 DEGs were viral genes and their expression in WSSV group either peaked at 18 hpi (and decreased at 56 hpi) or increased linearly up to 56 hpi, suggesting a different role played by these genes during the course of infection. The remaining DEGs showed that WSSV altered the expression of metabolic, immune, apoptotic and cytoskeletal genes and was able to inhibit NF-κB and JAK/STAT pathways. Interestingly, GE changes were not consistent through the course of infection but were dynamic with time, suggesting the complexity of host-pathogen interaction. These data offer novel insights into the cellular functions that are affected during the course of infection and ultimately provide a valuable resource towards our understanding of the host-pathogen dynamics and its variation with time.

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Grants

  1. BB/N005058/1/Biotechnology and Biological Sciences Research Council

MeSH Term

Animals
Aquaculture
Decapoda
Genes, Viral
Gills
Host-Pathogen Interactions
Immunity, Innate
Infections
Longitudinal Studies
Penaeidae
Transcriptome
White spot syndrome virus 1
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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