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Aug, 2019;6 (8): 190580.

A general fluorescent light-up probe for staining and quantifying protein.

Jiawei Zou, Gangyi Chen, Feng Du, Yi Yuan, Xin Huang, Juan Dong, Kexin Xie, Xin Cui, Zhuo Tang
Author Information
  1. Jiawei Zou: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  2. Gangyi Chen: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  3. Feng Du: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  4. Yi Yuan: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  5. Xin Huang: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  6. Juan Dong: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  7. Kexin Xie: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  8. Xin Cui: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.
  9. Zhuo Tang: Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China. ORCID
PMID: 31598246 DOI: 10.1098/rsos.190580

Abstract

Proteins are the primary functional agents in all cellular processes, facilitating various functions such as enzymes and structure-forming or signal-transducing molecules. In this work, we report a fluorescent dye, PyMDI-Zn, which could specifically bind with proteins and provide a red-shifted fluorescent emission. The visual analysis of protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis could be realized in 5 min by using PyMDI-Zn as a light-up dye. Based on its cell penetration and low toxicity, PyMDI-Zn could also be applied to locate protein-rich regions and organelles in live cell imaging. Moreover, the direct protein quantitation can be realized based on PyMDI-Zn, providing a method of screening for food adulteration by nitrogen-rich compounds.

Keywords

PyMDI-Zn SDS-PAGE live cell imaging protein quantitation

Associated Data

figshare | 10.6084/m9.figshare.c.4614731

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Journal Article
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Created with Highcharts 10.0.0PyMDI-Znproteinfluorescentcelldyerealizedlight-upliveimagingquantitationProteinsprimaryfunctionalagentscellularprocessesfacilitatingvariousfunctionsenzymesstructure-formingsignal-transducingmoleculesworkreportspecificallybindproteinsprovidered-shiftedemissionvisualanalysissodiumdodecylsulfate-polyacrylamidegelelectrophoresis5minusingBasedpenetrationlowtoxicityalsoappliedlocateprotein-richregionsorganellesMoreoverdirectcanbasedprovidingmethodscreeningfoodadulterationnitrogen-richcompoundsgeneralprobestainingquantifyingSDS-PAGE

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