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10 16, 2019;8 (10):

β-Endorphin Induction by Psychological Stress Promotes Leydig Cell Apoptosis through p38 MAPK Pathway in Male Rats.

Xiaofan Xiong, Lingyu Zhang, Meiyang Fan, Lin Han, Qiuhua Wu, Siyuan Liu, Jiyu Miao, Liying Liu, Xiaofei Wang, Bo Guo, Dongdong Tong, Lei Ni, Juan Yang, Chen Huang
Author Information
  1. Xiaofan Xiong: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  2. Lingyu Zhang: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  3. Meiyang Fan: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  4. Lin Han: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  5. Qiuhua Wu: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  6. Siyuan Liu: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  7. Jiyu Miao: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  8. Liying Liu: Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China, Xi'an 710061, China.
  9. Xiaofei Wang: Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China, Xi'an 710061, China.
  10. Bo Guo: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  11. Dongdong Tong: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  12. Lei Ni: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
  13. Juan Yang: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. yangjuan0112@xjtu.edu.cn.
  14. Chen Huang: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. hchen@xjtu.edu.cn.
PMID: 31623282 DOI: 10.3390/cells8101265

Abstract

Psychological stress (PS) disturbs the reproductive endocrine system and promotes male infertility, but the underlying pathogenic mechanisms have not been extensively studied. This study aimed to uncover the mechanisms of PS-induced male reproductive related abnormalities subjected to a 'terrified sound' exposure. Male rats subjected to PS displayed slow growth, decreased sperm quality, abnormal levels of the reproductive endocrine hormones, decreased expression of the reproductive-related proteins androgen-binding protein (ABP) and bromodomain-containing protein (BRDT), increased apoptosis in the testis, and accompanied by elevated levels of β-endorphin (β-EP). These effects were reversed by naloxone. Furthermore, PS-induced β-EP could promote mu opioid receptor (MOR) activation and ensure intracellular p38 MAPK phosphorylation and then lead to Leydig cells (LCs) apoptosis. The current result showed that β-EP was a key factor to PS-induced male infertility.

Keywords

Leydig cells p38 MAPK psychological stress sperm quality β-endorphin

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MeSH Term

Animals
Apoptosis
Blotting, Western
Cell Line
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunohistochemistry
In Situ Nick-End Labeling
Leydig Cells
Male
Phosphorylation
Rats
Rats, Sprague-Dawley
Sperm Count
Sperm Motility
Stress, Psychological
beta-Endorphin
p38 Mitogen-Activated Protein Kinases

Chemicals

beta-Endorphin
p38 Mitogen-Activated Protein Kinases
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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