OpenLB
Open Library of Bioscience
Advanced Search
International journal of molecular sciences
Oct 30, 2019;20 (21):

Soyuretox, an Intrinsically Disordered Polypeptide Derived from Soybean (Glycine Max) Ubiquitous Urease with Potential Use as a Biopesticide.

Karine Kappaun, Anne H S Martinelli, Valquiria Broll, Barbara Zambelli, Fernanda C Lopes, Rodrigo Ligabue-Braun, Leonardo L Fruttero, Natalia R Moyetta, Carla D Bonan, Celia R Carlini, Stefano Ciurli
Author Information
  1. Karine Kappaun: Graduate Program in Medicine and Health Sciences, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, RS, Brazil. karine.kappaun@gmail.com.
  2. Anne H S Martinelli: Department of Biophysics and Center of Biotechnology, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre 91501-970, RS, Brazil. ahsmartinelli@yahoo.com.br.
  3. Valquiria Broll: Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre 91501-970, RS, Brazil. valbroll@gmail.com. ORCID
  4. Barbara Zambelli: Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy. barbara.zambelli@unibo.it. ORCID
  5. Fernanda C Lopes: Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre 91501-970, RS, Brazil. fernandacortezlopes@gmail.com. ORCID
  6. Rodrigo Ligabue-Braun: Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre 91501-970, RS, Brazil. ligabue.braun@gmail.com.
  7. Leonardo L Fruttero: Graduate Program in Medicine and Health Sciences, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, RS, Brazil. leonardofruttero@gmail.com.
  8. Natalia R Moyetta: Department of Clinical Biochemistry, CIBICI-CONICET, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Córdoba 5000, Argentina. nataliarmoyetta@gmail.com.
  9. Carla D Bonan: Department of Cellular and Molecular Biology, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 91501-970, RS, Brazil. cbonan@pucrs.br. ORCID
  10. Celia R Carlini: Graduate Program in Medicine and Health Sciences, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, RS, Brazil. celia.carlini@pucrs.br. ORCID
  11. Stefano Ciurli: Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy. stefano.ciurli@unibo.it. ORCID
PMID: 31671552 DOI: 10.3390/ijms20215401

Abstract

Ureases from different biological sources display non-ureolytic properties that contribute to plant defense, in addition to their classical enzymatic urea hydrolysis. Antifungal and entomotoxic effects were demonstrated for Jaburetox, an intrinsically disordered polypeptide derived from jack bean () urease. Here we describe the properties of Soyuretox, a polypeptide derived from soybean () ubiquitous urease. Soyuretox was fungitoxic to , leading to the production of reactive oxygen species. Soyuretox further induced aggregation of hemocytes, indicating an interference on the insect immune response. No relevant toxicity of Soyuretox to zebrafish larvae was observed. These data suggest the presence of antifungal and entomotoxic portions of the amino acid sequences encompassing both Soyuretox and Jaburetox, despite their small sequence identity. Nuclear Magnetic Resonance (NMR) and circular dichroism (CD) spectroscopic data revealed that Soyuretox, in analogy with Jaburetox, possesses an intrinsic and largely disordered nature. Some folding is observed upon interaction of Soyuretox with sodium dodecyl sulfate (SDS) micelles, taken here as models for membranes. This observation suggests the possibility for this protein to modify its secondary structure upon interaction with the cells of the affected organisms, leading to alterations of membrane integrity. Altogether, Soyuretox can be considered a promising biopesticide for use in plant protection.

Keywords

antifungal entomotoxin circular dichroism (CD) intrinsically disordered proteins molecular dynamics (MD) nuclear magnetic resonance (NMR)

References

  1. Behav Brain Res. 2016 Sep 15;311:368-374 [PMID: 27247142]
  2. J Insect Physiol. 2009 Mar;55(3):255-63 [PMID: 19121321]
  3. J Chem Theory Comput. 2008 Mar;4(3):435-47 [PMID: 26620784]
  4. Biopolymers. 2012;98(4):367-84 [PMID: 23193601]
  5. J Econ Entomol. 1997 Apr;90(2):340-8 [PMID: 9145031]
  6. Adv Exp Med Biol. 2015;870:49-122 [PMID: 26387100]
  7. Trends Neurosci. 2014 May;37(5):264-78 [PMID: 24726051]
  8. Mol Syst Biol. 2011 Oct 11;7:539 [PMID: 21988835]
  9. Appl Biochem Biotechnol. 2016 Dec;180(8):1542-1558 [PMID: 27544774]
  10. Toxins (Basel). 2012 Feb;4(2):55-67 [PMID: 22474566]
  11. World J Microbiol Biotechnol. 2016 Sep;32(9):144 [PMID: 27430511]
  12. Annu Rev Biophys Biomol Struct. 2000;29:291-325 [PMID: 10940251]
  13. J Comput Chem. 2004 Oct;25(13):1605-12 [PMID: 15264254]
  14. Anal Biochem. 1976 May 7;72:248-54 [PMID: 942051]
  15. Science. 1994 Feb 11;263(5148):777-84 [PMID: 8303294]
  16. Acc Chem Res. 2011 Jul 19;44(7):520-30 [PMID: 21542631]
  17. Peptides. 2007 Oct;28(10):2042-50 [PMID: 17875343]
  18. Prog Neuropsychopharmacol Biol Psychiatry. 2011 Aug 1;35(6):1409-15 [PMID: 20837077]
  19. Prog Neuropsychopharmacol Biol Psychiatry. 2014 Dec 3;55:94-100 [PMID: 24690524]
  20. Biochim Biophys Acta. 2006 Sep;1758(9):1483-98 [PMID: 16987495]
  21. Colloids Surf B Biointerfaces. 2017 Nov 1;159:849-860 [PMID: 28892869]
  22. J Neurosci. 2010 Apr 28;30(17):6048-57 [PMID: 20427663]
  23. Toxicon. 2016 Feb;110:90-109 [PMID: 26690979]
  24. J Mol Biol. 1982 May 5;157(1):105-32 [PMID: 7108955]
  25. Nature. 1992 Mar 5;356(6364):83-5 [PMID: 1538787]
  26. Toxicon. 2013 Sep;71:76-83 [PMID: 23726854]
  27. Arch Insect Biochem Physiol. 2000 Aug;44(4):162-71 [PMID: 10918311]
  28. Chem Rev. 2014 Apr 23;114(8):4206-28 [PMID: 24369791]
  29. Plant Cell Rep. 2018 Jun;37(6):923-932 [PMID: 29532251]
  30. Biochim Biophys Acta. 2014 Mar;1840(3):935-44 [PMID: 24239686]
  31. Brain Res Bull. 2011 Nov 25;86(5-6):385-9 [PMID: 21907764]
  32. Toxicology. 2006 Sep 21;226(2-3):229-37 [PMID: 16930798]
  33. Naturwissenschaften. 2013 May;100(5):459-67 [PMID: 23619940]
  34. PLoS One. 2014 Sep 04;9(9):e105862 [PMID: 25187954]
  35. FEBS J. 2015 Mar;282(6):1043-64 [PMID: 25605001]
  36. Insect Biochem Mol Biol. 2008 Nov;38(11):1023-32 [PMID: 18952169]
  37. Int J Mol Sci. 2016 Oct 18;17(10): [PMID: 27763554]
  38. Neurotoxicology. 2012 Jun;33(3):469-75 [PMID: 22459995]
  39. Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3841-6 [PMID: 24567386]
  40. J Mol Biol. 2010 Jul 16;400(3):274-83 [PMID: 20471401]
  41. Biochim Biophys Acta. 2009 Dec;1794(12):1848-54 [PMID: 19751848]
  42. Plant Cell Rep. 2016 May;35(5):1189-203 [PMID: 26956134]
  43. Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11504-9 [PMID: 8876165]
  44. J Comput Chem. 2004 Oct;25(13):1656-76 [PMID: 15264259]
  45. Biochim Biophys Acta. 2015 Oct;1848(10 Pt A):2365-73 [PMID: 26209560]
  46. J Adv Res. 2018 May 28;13:3-17 [PMID: 30094078]
  47. Toxicon. 2005 May;45(6):753-60 [PMID: 15804524]
  48. Biochim Biophys Acta. 2015 Feb;1850(2):255-62 [PMID: 25445710]
  49. Toxicon. 2014 May;82:18-25 [PMID: 24561121]
  50. Eur J Biochem. 2004 Apr;271(7):1357-63 [PMID: 15030486]
  51. Neurobiol Learn Mem. 2012 Nov;98(4):321-8 [PMID: 23044456]
  52. Biochim Biophys Acta. 2011 Dec;1810(12):1246-51 [PMID: 21875650]
  53. Parasit Vectors. 2016 Jul 25;9(1):412 [PMID: 27455853]
  54. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 [PMID: 388439]
  55. Curr Microbiol. 2011 Apr;62(4):1209-17 [PMID: 21170711]
  56. Peptides. 2012 Nov;38(1):22-32 [PMID: 22922160]
  57. Nature. 2013 Apr 25;496(7446):498-503 [PMID: 23594743]
  58. Insect Biochem Mol Biol. 2011 Jun;41(6):388-99 [PMID: 21377528]
  59. Antimicrob Agents Chemother. 2018 May 25;62(6): [PMID: 29610196]
  60. Colloids Surf B Biointerfaces. 2016 Sep 1;145:576-585 [PMID: 27281243]

Grants

  1. 47.5908/2012/Conselho Nacional de Desenvolvimento Científico e Tecnológico
  2. 47.5908/2012/Conselho Nacional de Desenvolvimento Científico e Tecnológico

MeSH Term

Animals
Biological Control Agents
Candida albicans
Circular Dichroism
Hemocytes
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Dynamics Simulation
Peptides
Plant Proteins
Protein Folding
Reactive Oxygen Species
Rhodnius
Glycine max
Urease

Chemicals

Biological Control Agents
Peptides
Plant Proteins
Reactive Oxygen Species
Urease
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0SoyuretoxJaburetoxdisorderedpropertiesplantentomotoxicintrinsicallypolypeptidederivedureaseleadingobserveddataantifungalNMRcirculardichroismCDuponinteractionUreasesdifferentbiologicalsourcesdisplaynon-ureolyticcontributedefenseadditionclassicalenzymaticureahydrolysisAntifungaleffectsdemonstratedjackbeandescribesoybeanubiquitousfungitoxicproductionreactiveoxygenspeciesinducedaggregationhemocytesindicatinginterferenceinsectimmuneresponserelevanttoxicityzebrafishlarvaesuggestpresenceportionsaminoacidsequencesencompassingdespitesmallsequenceidentityNuclearMagneticResonancespectroscopicrevealedanalogypossessesintrinsiclargelynaturefoldingsodiumdodecylsulfateSDSmicellestakenmodelsmembranesobservationsuggestspossibilityproteinmodifysecondarystructurecellsaffectedorganismsalterationsmembraneintegrityAltogethercanconsideredpromisingbiopesticideuseprotectionIntrinsicallyDisorderedPolypeptideDerivedSoybeanGlycineMaxUbiquitousUreasePotentialUseBiopesticideentomotoxinproteinsmoleculardynamicsMDnuclearmagneticresonance

Similar Articles

Cited By (1)