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BMC genomics
Nov 06, 2019;20 (1): 807.

RNA-seq analysis provides insights into cold stress responses of Xanthomonas citri pv. citri.

Jin-Xing Liao, Kai-Huai Li, Jin-Pei Wang, Jia-Ru Deng, Qiong-Guang Liu, Chang-Qing Chang
Author Information
  1. Jin-Xing Liao: Integrative Microbiology Research Centre, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China.
  2. Kai-Huai Li: Integrative Microbiology Research Centre, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China.
  3. Jin-Pei Wang: Integrative Microbiology Research Centre, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China.
  4. Jia-Ru Deng: Integrative Microbiology Research Centre, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China.
  5. Qiong-Guang Liu: Integrative Microbiology Research Centre, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China.
  6. Chang-Qing Chang: Integrative Microbiology Research Centre, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China. changcq@scau.edu.cn.
PMID: 31694530 DOI: 10.1186/s12864-019-6193-0

Abstract

BACKGROUND: Xanthomonas citri pv. citri (Xcc) is a citrus canker causing Gram-negative bacteria. Currently, little is known about the biological and molecular responses of Xcc to low temperatures.
RESULTS: Results depicted that low temperature significantly reduced growth and increased biofilm formation and unsaturated fatty acid (UFA) ratio in Xcc. At low temperature Xcc formed branching structured motility. Global transcriptome analysis revealed that low temperature modulates multiple signaling networks and essential cellular processes such as carbon, nitrogen and fatty acid metabolism in Xcc. Differential expression of genes associated with type IV pilus system and pathogenesis are important cellular adaptive responses of Xcc to cold stress.
CONCLUSIONS: Study provides clear insights into biological characteristics and genome-wide transcriptional analysis based molecular mechanism of Xcc in response to low temperature.

Keywords

Biofilm formation Fatty acids Low temperature stress Metabolism Motility Xanthomonas

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Grants

  1. 2015CB150600/The National Basic Research Program of China

MeSH Term

Cold-Shock Response
Flagella
Gene Expression Profiling
Membrane Lipids
RNA-Seq
Xanthomonas

Chemicals

Membrane Lipids
Journal Article
Article has an altmetric score of 2

Word Cloud

Created with Highcharts 10.0.0XcclowtemperaturecitriXanthomonasresponsesanalysisstresspvbiologicalmolecularformationfattyacidcellularcoldprovidesinsightsBACKGROUND:citruscankercausingGram-negativebacteriaCurrentlylittleknowntemperaturesRESULTS:ResultsdepictedsignificantlyreducedgrowthincreasedbiofilmunsaturatedUFAratioformedbranchingstructuredmotilityGlobaltranscriptomerevealedmodulatesmultiplesignalingnetworksessentialprocessescarbonnitrogenmetabolismDifferentialexpressiongenesassociatedtypeIVpilussystempathogenesisimportantadaptiveCONCLUSIONS:Studyclearcharacteristicsgenome-widetranscriptionalbasedmechanismresponseRNA-seqBiofilmFattyacidsLowMetabolismMotility

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