Molecular detection of Campylobacter jejuni in patients with Crohn's disease in Iran.
Akram Sarabi Asiabar, Hamid Asadzadeh Aghdaei, Samin Zamani, Saied Bokaie, Mohammad Reza Zali, Mohammad Mehdi Feizabadi
Author Information
Akram Sarabi Asiabar: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Hamid Asadzadeh Aghdaei: Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Samin Zamani: Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Saied Bokaie: Department of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Mohammad Reza Zali: Department of Inflammatory Bowel Disease, Research Center for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad Mehdi Feizabadi: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Crohn's disease is one of the most significant intestinal disorders and is known as inflammatory bowel disease; spp. are one of the leading causes of bacterial gastroenteritis in humans. In this study, 60 tissue samples, including 30 cases with Crohn's disease and 30 cases with no inflammatory bowel disease, were collected. Patients were referred to Taleghani hospital and Behboud clinic between March 2015 and May 2016. Biopsies were used for DNA extraction and assessment of in patients with Crohn's disease and controls using polymerase chain reaction and quantitative real-time polymerase chain reaction. All positive amplified fragments were sequenced. The gene encoding 16S rRNA, specific to genus, was amplified. The results were positive for genus in patients with Crohn's disease compared to healthy individuals. The quantitative real-time PCR showed a significantly higher prevalence of Campylobacter jejuni, particularly in hippurate hydrolase in tissue specimens of patients with Crohn's disease compared to control group. The correlation between and diarrhea symptoms in patients with Crohn's disease and controls was investigated. One positive case of found in patients without diarrhea was compared with 13 patients with diarrhea. The present study demonstrated the alarmingly high rate of prevalence in Crohn's disease patients with diarrhea symptoms. However, further investigation is needed to determine the possible causing factors of this disease.
