Mohsen Gozari, Nima Bahador, Mohammad Seddiq Mortazavi, Ebrahim Eftekhar, Amir Reza Jassbi
We isolated an actinobacterium, sp. strain SP 85 from the marine sponge Polyphasic identification of the microorganism showed that the strain SP 85 had high 16S rRNA gene similarity (99%) with strain NBRC 12805, while some physiological and biochemical differences were observed. A cytotoxic compound, was isolated from the active culture extract of the strain SP 85 by bioassay-guided purification over silica gel column chromatography, preparative TLC, and HPLC. The structure elucidation based on the spectroscopic analysis, including UV, ESI-MS, and C NMR data revealed that compound is an analog of anti-tumor drug, "olivomycin A". The compound showed high cytotoxic activity against three human cancer cell lines, including SW480, HepG2, and MCF7 with IC values of 16, 93, and 78 nM, respectively. exhibited significantly (2-10 times) higher cytotoxicity against the tumor cell lines in comparison with HUVECs as the normal cell line, which also induced apoptosis in the tested cancerous cell line. This is the first report on the production of an "olivomycin A" derivative by a sponge-associated , showing the great potential of sponge-associated actinobacteria in producing cytotoxic natural products.
