Methylations in complex carbapenem biosynthesis are catalyzed by a single cobalamin-dependent radical S-adenosylmethionine enzyme.
Erica K Sinner, Michael S Lichstrahl, Rongfeng Li, Daniel R Marous, Craig A Townsend
Author Information
Erica K Sinner: Department of Chemistry, Johns Hopkins University, 3400 N Charles St., Baltimore, MD 21218, USA. ctownsend@jhu.edu.
Complex carbapenem β-lactam antibiotics contain diverse C6 alkyl substituents constructed by cobalamin-dependent radical SAM enzymes. TokK installs the C6 isopropyl chain found in asparenomycin. Time-course analyses of TokK and its ortholog ThnK, which forms the C6 ethyl chain of thienamycin, indicate that catalysis occurs through a sequence of discrete, non-processive methyl transfers.
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R01 AI014937/NIAID NIH HHS
R01 AI121072/NIAID NIH HHS
R56 AI014937/NIAID NIH HHS
T32 GM080189/NIGMS NIH HHS
Anti-Bacterial Agents
Carbapenems
Catalysis
Methionine Adenosyltransferase
Methylation
Vitamin B 12
Anti-Bacterial Agents
Carbapenems
Methionine Adenosyltransferase
Vitamin B 12
