Borregowda Nandini: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru 560 006, Karnataka, India.
Hariprasad Puttaswamy: Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi 110 016, India.
Prakash H S: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru 560 006, Karnataka, India.
Shivakantkumar Adhikari: Laboratory of Plant Healthcare and Diagnostics, PG Department of Biotechnology and Microbiology, Karnatak University, Dharwad 580 003, India.
Sudisha Jogaiah: Laboratory of Plant Healthcare and Diagnostics, PG Department of Biotechnology and Microbiology, Karnatak University, Dharwad 580 003, India.
Geetha Nagaraja: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru 560 006, Karnataka, India.
Nanoemulsion was formulated from membrane lipids of spp. with the non-ionic surfactant Tween 80 by the ultrasonic emulsification method. Nanoemulsion with a droplet diameter of 5 to 51 nm was obtained. The possible effects of membrane lipid nanoemulsion on pearl millet (PM) seed growth parameters and elicitation of downy mildew (DM) disease resistance in PM was analyzed to develop an eco-friendly disease management strategy. Seed priming with nanoemulsion illustrates significant protection and elevated levels of early defense gene expression. Lipid profiling of Trichoderma spp. reveals the presence of oleic acid as a major fatty acid molecule. The prominent molecule in the purified lipid fraction of T. brevicompactum (UP-91) responsible for the elicitation of induction of systemic resistance in PM host against DM pathogen was predicted as (E)-N-(1, 3-dihydroxyoctadec-4-en-2yl) acetamide. The results suggest that protection offered by the novel nanoemulsion formulation is systemic in nature and durable and offers a newer sustainable approach to manage biotrophic oomycetous pathogen.
