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Frontiers in neurology
2019;10 1289.

SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury.

Qiannan Ren, Zhiying Hu, Yuting Jiang, Xiaoning Tan, Benson O A Botchway, Nashwa Amin, Gaoping Lin, Yu Geng, Marong Fang
Author Information
  1. Qiannan Ren: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
  2. Zhiying Hu: Department of Obstetrics and Gynecology, Hangzhou Red Cross Hospital, Hangzhou, China.
  3. Yuting Jiang: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
  4. Xiaoning Tan: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
  5. Benson O A Botchway: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
  6. Nashwa Amin: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
  7. Gaoping Lin: Department of Neurology, Zhejiang Provincial People's Hospital, Hangzhou, China.
  8. Yu Geng: Department of Neurology, Zhejiang Provincial People's Hospital, Hangzhou, China.
  9. Marong Fang: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
PMID: 31920915 DOI: 10.3389/fneur.2019.01289

Abstract

Silent information regulator 1 (SIRT1) contributes to cellular regulation. Previous studies have reported SIRT1 to be abnormally expressed in the ischemic penumbra of cerebral ischemia/reperfusion (I/R) injury rat model. We investigated the effect of SIRT1 on oxygen and glucose deprivation/reperfusion (OGD/R) cell injury. Over-expressed or silenced SIRT1 pheochromocytoma 12 (PC12) cells were exposed to an OGD/R injury. Western blot, TUNEL staining and immunofluorescence analyses were performed to assess apoptosis and autophagy. We found autophagy and apoptosis to be up-regulated and down-regulated, respectively, following the over-expression of SIRT1 in the OGD/R-induced PC12 cells. We also found the silencing of SIRT1 to culminate in the down-regulation and up-regulation of autophagy and apoptosis, respectively. On the basis of our results, we surmise that SIRT1 can promote autophagy and inhibit apoptosis , and thus exhibit potential neuroprotection against OGD/R-induced injury. This could facilitate in the development of therapeutic approaches for cerebral I/R injury.

Keywords

apoptosis autophagy cerebral ischemia/reperfusion (I/R) injury oxygen and glucose deprivation/reperfusion (OGD/R) silent information regulator 1(SIRT1)

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Journal Article
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