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Journal of developmental biology
Jan 31, 2020;8 (1):

Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish Embryos.

Christopher D Small, Megan El-Khoury, Ghislain Deslongchamps, Tillmann J Benfey, Bryan D Crawford
Author Information
  1. Christopher D Small: Biology Department, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.
  2. Megan El-Khoury: Biology Department, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.
  3. Ghislain Deslongchamps: Chemistry Department, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.
  4. Tillmann J Benfey: Biology Department, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.
  5. Bryan D Crawford: Biology Department, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.
PMID: 32023839 DOI: 10.3390/jdb8010003

Abstract

Hypoxia induces precocious hatching in zebrafish, but we do not have a clear understanding of the molecular mechanisms regulating the activation of the hatching enzyme or how these mechanisms trigger precocious hatching under unfavorable environmental conditions. Using immunohistochemistry, pharmacological inhibition of matrix metalloproteinase 13 (Mmp13), and in vivo zymography, we show that Mmp13a is present in the hatching gland just as embryos become hatching competent and that Mmp13a activity is required for both normal hatching and Hypoxia-induced precocious hatching. We conclude that Mmp13a likely functions in activating the hatching enzyme zymogen and that Mmp13a activity is necessary but not sufficient for hatching in zebrafish. This study highlights the broad nature of MMP function in development and provides a non-mammalian example of extra-embryonic processes mediated by MMP activity.

Keywords

developmental trade-off hatching hypoxia in vivo zymography matrix metalloproteinase 13 (Mmp13) stress zebrafish

References

  1. Eur Respir J. 2011 Jul;38(1):191-208 [PMID: 21177845]
  2. Mol Cancer Ther. 2018 Jun;17(6):1147-1155 [PMID: 29735645]
  3. Conf Proc IEEE Eng Med Biol Soc. 2004;2004:5061-4 [PMID: 17271455]
  4. J Exp Biol. 2004 Feb;207(Pt 5):853-68 [PMID: 14747416]
  5. Dev Dyn. 1995 Jul;203(3):253-310 [PMID: 8589427]
  6. Dev Reprod. 2017 Dec;21(4):351-359 [PMID: 29359200]
  7. R Soc Open Sci. 2019 Jan 30;6(1):181020 [PMID: 30800355]
  8. Zebrafish. 2009 Dec;6(4):347-54 [PMID: 19929220]
  9. J Exp Biol. 2007 Apr;210(Pt 8):1435-45 [PMID: 17401126]
  10. Toxicol Sci. 2007 Nov;100(1):168-79 [PMID: 17728286]
  11. Annu Rev Anim Biosci. 2018 Feb 15;6:47-68 [PMID: 29447475]
  12. Dev Biol. 1992 Oct;153(2):250-8 [PMID: 1397682]
  13. PLoS One. 2011;6(5):e19683 [PMID: 21625559]
  14. Cardiovasc Res. 2006 Feb 15;69(3):562-73 [PMID: 16405877]
  15. Chem Biol. 2005 Feb;12(2):181-9 [PMID: 15734645]
  16. Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1240-5 [PMID: 15644436]
  17. Hum Reprod. 1998 Oct;13(1O):2926-32 [PMID: 9804257]
  18. Trends Genet. 2015 Jul;31(7):402-10 [PMID: 25999217]
  19. J Diabetes Complications. 2018 Mar;32(3):249-257 [PMID: 29306589]
  20. J Dev Biol. 2019 Dec 03;7(4): [PMID: 31816958]
  21. Int J Mol Sci. 2019 Aug 07;20(16): [PMID: 31394726]
  22. BMC Evol Biol. 2014 Oct 19;14:221 [PMID: 25326699]
  23. Biol Rev Camb Philos Soc. 2008 Feb;83(1):13-34 [PMID: 18093234]
  24. Cold Spring Harb Perspect Biol. 2011 Dec 01;3(12): [PMID: 21917992]
  25. Zoolog Sci. 1996 Jun;13(3):331-40 [PMID: 8987518]
  26. Nat Methods. 2012 Jun 28;9(7):676-82 [PMID: 22743772]
  27. Comp Biochem Physiol A Mol Integr Physiol. 2018 Dec;226:49-56 [PMID: 30114471]
  28. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7331-5 [PMID: 11404478]
  29. Histochem Cell Biol. 2018 Mar;149(3):277-286 [PMID: 29350268]
  30. J Anim Ecol. 2011 Jul;80(4):844-53 [PMID: 21401593]
  31. Mol Genet Genomics. 2014 Dec;289(6):1045-60 [PMID: 25092473]
  32. Hum Reprod Update. 2002 Jul-Aug;8(4):333-43 [PMID: 12206468]
  33. Biochim Biophys Acta Mol Cell Res. 2017 Nov;1864(11 Pt A):1940-1951 [PMID: 28456643]
  34. FEBS J. 2008 Dec;275(23):5934-46 [PMID: 19021768]
  35. Am J Reprod Immunol. 2016 Mar;75(3):208-17 [PMID: 26706391]
  36. Reprod Med Biol. 2015 Aug 15;15(2):53-58 [PMID: 29259421]
  37. J Exp Biol. 2018 Jan 11;221(Pt 1): [PMID: 29326114]
  38. Curr Top Dev Biol. 2018;130:245-274 [PMID: 29853179]
  39. Neural Regen Res. 2016 Mar;11(3):357-62 [PMID: 27127457]
  40. Curr Top Dev Biol. 2018;130:275-305 [PMID: 29853180]
  41. Proc Biol Sci. 2014 Jul 7;281(1786): [PMID: 24850928]
  42. Biomedicines. 2019 Nov 29;7(4): [PMID: 31795436]
  43. Zoolog Sci. 2016 Jun;33(3):272-81 [PMID: 27268981]
  44. Dev Biol. 2005 Oct 15;286(2):405-14 [PMID: 16112664]
  45. J Hum Reprod Sci. 2013 Jul;6(3):183-8 [PMID: 24347932]
  46. Matrix Biol. 2011 Apr;30(3):169-77 [PMID: 21292002]
  47. Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):E2189-98 [PMID: 27035978]
  48. J Mol Biol. 2010 Oct 8;402(5):865-78 [PMID: 20727360]
  49. Zebrafish. 2007 Spring;4(1):21-40 [PMID: 18041940]
  50. BMC Evol Biol. 2010 Aug 27;10:260 [PMID: 20796321]
  51. Dev Biol. 1992 Feb;149(2):349-56 [PMID: 1730389]
  52. Curr Top Dev Biol. 2016;117:275-88 [PMID: 26969983]

Grants

  1. 341540-2013; 2018-04559/Natural Sciences and Engineering Research Council of Canada
Journal Article
Article has an altmetric score of 3

Word Cloud

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