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Microbial cell factories
Feb 11, 2020;19 (1): 27.

Effect of the TetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona.

Haocheng He, Shuangqin Yuan, Jinjuan Hu, Jianming Chen, Jie Rang, Jianli Tang, Zhudong Liu, Ziyuan Xia, Xuezhi Ding, Shengbiao Hu, Liqiu Xia
Author Information
  1. Haocheng He: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  2. Shuangqin Yuan: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  3. Jinjuan Hu: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  4. Jianming Chen: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  5. Jie Rang: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  6. Jianli Tang: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  7. Zhudong Liu: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  8. Ziyuan Xia: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  9. Xuezhi Ding: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  10. Shengbiao Hu: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
  11. Liqiu Xia: Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China. xialq@hunnu.edu.cn. ORCID
PMID: 32046731 DOI: 10.1186/s12934-020-01299-z

Abstract

BACKGROUND: Saccharopolyspora pogona is a prominent industrial strain due to its production of butenyl-spinosyn, a high-quality insecticide against a broad spectrum of insect pests. TetR family proteins are diverse in a tremendous number of microorganisms and some are been researched to have a key role in metabolic regulation. However, specific functions of TetR family proteins in S. pogona are yet to characterize.
RESULTS: In the present study, the overexpression of the tetR-like gene sp1418 in S. pogona resulted in marked effects on vegetative growth, sporulation, butenyl-spinosyn biosynthesis, and oxidative stress. By using qRT-PCR analysis, mass spectrometry, enzyme activity detection, and sp1418 knockout verification, we showed that most of these effects could be attributed to the overexpression of Sp1418, which modulated enzymes related to the primary metabolism, oxidative stress and secondary metabolism, and thereby resulted in distinct growth characteristics and an unbalanced supply of precursor monomers for butenyl-spinosyn biosynthesis.
CONCLUSION: This study revealed the function of Sp1418 and enhanced the understanding of the metabolic network in S. pogona, and provided insights into the improvement of secondary metabolite production.

Keywords

Butenyl-spinosyn Oxidative stress Saccharopolyspora pogona TetR family transcriptional regulator

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Grants

  1. 31770106/the National Natural Science Foundation of China
  2. 2012CB722301/National Basic Research Program of China (973 Program)
  3. 20134486/the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province

MeSH Term

Bacterial Proteins
Gene Expression Regulation, Bacterial
Genetic Engineering
Metabolic Networks and Pathways
Saccharopolyspora

Chemicals

Bacterial Proteins
Journal Article

Word Cloud

Created with Highcharts 10.0.0pogonaTetRfamilySaccharopolysporabutenyl-spinosynmetabolicSstressSp1418productionproteinsstudyoverexpressionsp1418resultedeffectsgrowthbiosynthesisoxidativemetabolismsecondarynetworktranscriptionalregulatorBACKGROUND:prominentindustrialstrainduehigh-qualityinsecticidebroadspectruminsectpestsdiversetremendousnumbermicroorganismsresearchedkeyroleregulationHoweverspecificfunctionsyetcharacterizeRESULTS:presenttetR-likegenemarkedvegetativesporulationusingqRT-PCRanalysismassspectrometryenzymeactivitydetectionknockoutverificationshowedattributedmodulatedenzymesrelatedprimarytherebydistinctcharacteristicsunbalancedsupplyprecursormonomersCONCLUSION:revealedfunctionenhancedunderstandingprovidedinsightsimprovementmetaboliteEffectglobalButenyl-spinosynOxidative

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