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02 11, 2020;10 (1): 2347.

A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery.

Khrongkhwan Thammatinna, MacKennon E Egan, Htut Htut Htoo, Kanika Khanna, Joseph Sugie, Jason F Nideffer, Elizabeth Villa, Anchalee Tassanakajon, Joe Pogliano, Poochit Nonejuie, Vorrapon Chaikeeratisak
Author Information
  1. Khrongkhwan Thammatinna: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
  2. MacKennon E Egan: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
  3. Htut Htut Htoo: Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand. ORCID
  4. Kanika Khanna: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
  5. Joseph Sugie: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
  6. Jason F Nideffer: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
  7. Elizabeth Villa: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA. ORCID
  8. Anchalee Tassanakajon: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
  9. Joe Pogliano: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
  10. Poochit Nonejuie: Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand.
  11. Vorrapon Chaikeeratisak: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. vorrapon.c@chula.ac.th. ORCID
PMID: 32047244 DOI: 10.1038/s41598-020-59396-3

Abstract

Since the emergence of deadly pathogens and multidrug-resistant bacteria at an alarmingly increased rate, bacteriophages have been developed as a controlling bioagent to prevent the spread of pathogenic bacteria. One of these pathogens, disease-causing Vibrio parahaemolyticus (VP) which induces acute hepatopancreatic necrosis, is considered one of the deadliest shrimp pathogens, and has recently become resistant to various classes of antibiotics. Here, we discovered a novel vibriophage that specifically targets the vibrio host, VP. The vibriophage, designated Seahorse, was classified in the family Siphoviridae because of its icosahedral capsid surrounded by head fibers and a non-contractile long tail. Phage Seahorse was able to infect the host in a broad range of pH and temperatures, and it had a relatively short latent period (nearly 30 minutes) in which it produced progeny at 72 particles per cell at the end of its lytic cycle. Upon phage infection, the host nucleoid condensed and became toroidal, similar to the bacterial DNA morphology seen during tetracycline treatment, suggesting that phage Seahorse hijacked host biosynthesis pathways through protein translation. As phage Seahorse genome encodes 48 open reading frames with many hypothetical proteins, this genome could be a potential untapped resource for the discovery of phage-derived therapeutic proteins.

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Grants

  1. R01 GM033050/NIGMS NIH HHS
  2. R01 GM129245/NIGMS NIH HHS

MeSH Term

Bacteria
Bacteriophages
Chromosomes, Bacterial
Genome, Viral
Host Specificity
Microbial Viability
Protein Biosynthesis
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't
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Word Cloud

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