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Nature neuroscience
03, 2020;23 (3): 363-374.

Functionally distinct subgroups of oligodendrocyte precursor cells integrate neural activity and execute myelin formation.

Roberta Marisca, Tobias Hoche, Eneritz Agirre, Laura Jane Hoodless, Wenke Barkey, Franziska Auer, Gonçalo Castelo-Branco, Tim Czopka
Author Information
  1. Roberta Marisca: Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  2. Tobias Hoche: Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  3. Eneritz Agirre: Laboratory of Molecular Neurobiology, Department Medical Biochemistry and Biophysics, Biomedicum, Karolinska Institutet, Stockholm, Sweden. ORCID
  4. Laura Jane Hoodless: Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  5. Wenke Barkey: Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  6. Franziska Auer: Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  7. Gonçalo Castelo-Branco: Laboratory of Molecular Neurobiology, Department Medical Biochemistry and Biophysics, Biomedicum, Karolinska Institutet, Stockholm, Sweden. ORCID
  8. Tim Czopka: Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany. Tim.Czopka@tum.de. ORCID
PMID: 32066987 DOI: 10.1038/s41593-019-0581-2

Abstract

Recent reports have revealed that oligodendrocyte precursor cells (OPCs) are heterogeneous. It remains unclear whether such heterogeneity reflects different subtypes of cells with distinct functions or instead reflects transiently acquired states of cells with the same function. By integrating lineage formation of individual OPC clones, single-cell transcriptomics, calcium imaging and neural activity manipulation, we show that OPCs in the zebrafish spinal cord can be divided into two functionally distinct groups. One subgroup forms elaborate networks of processes and exhibits a high degree of calcium signaling, but infrequently differentiates despite contact with permissive axons. Instead, these OPCs divide in an activity- and calcium-dependent manner to produce another subgroup, with higher process motility and less calcium signaling and that readily differentiates. Our data show that OPC subgroups are functionally diverse in their response to neurons and that activity regulates the proliferation of a subset of OPCs that is distinct from the cells that generate differentiated oligodendrocytes.

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Grants

  1. 681893/European Research Council
  2. 714440/European Research Council

MeSH Term

Animals
Animals, Genetically Modified
Calcium Signaling
Cell Differentiation
Cell Division
Cell Lineage
Cell Proliferation
Embryo, Nonmammalian
Myelin Sheath
Nerve Net
Oligodendrocyte Precursor Cells
Spinal Cord
Swimming
Zebrafish
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 45

Word Cloud

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