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03 05, 2020;10 (1): 4115.

A Rationale for the Use of Clotted Vertebral Bone Marrow to Aid Tissue Regeneration Following Spinal Surgery.

F Salamanna, D Contartese, G Giavaresi, L Sicuro, G Barbanti Brodano, A Gasbarrini, M Fini
Author Information
  1. F Salamanna: Laboratory of Preclinical and Surgical Studies, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy. francesca.salamanna@ior.it. ORCID
  2. D Contartese: Laboratory of Preclinical and Surgical Studies, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
  3. G Giavaresi: Laboratory of Preclinical and Surgical Studies, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
  4. L Sicuro: Laboratory of Preclinical and Surgical Studies, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy. ORCID
  5. G Barbanti Brodano: Department of Oncological and Degenerative Spine Surgery, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
  6. A Gasbarrini: Department of Oncological and Degenerative Spine Surgery, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
  7. M Fini: Laboratory of Preclinical and Surgical Studies, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
PMID: 32139727 DOI: 10.1038/s41598-020-60934-2

Abstract

Vertebral body bone marrow aspirate (V-BMA), easily accessible simultaneously with the preparation of the site for pedicle screw insertion during spinal procedures, is becoming an increasingly used cell therapy approach in spinal surgery. However, the main drawbacks for V-BMA use are the lack of a standardized procedure and of a structural texture with the possibility of diffusion away from the implant site. The aim of this study was to evaluate, characterize and compare the biological characteristics of MSCs from clotted V-BMA and MSCs from whole and concentrate V-BMAs. MSCs from clotted V-BMA showed the highest cell viability and growth factors expression (TGF-β, VEGF-A, FGF2), the greatest colony forming unit (CFU) potency, cellular homogeneity, ability to differentiate towards the osteogenic (COL1AI, TNFRSF11B, BGLAP) and chondrogenic phenotype (SOX9) and the lowest ability to differentiate toward the adipogenic lineage (ADIPOQ) in comparison to all the other culture conditions. Additionally, results revealed that MSCs, differently isolated, expressed different level of HOX and TALE signatures and that PBX1 and MEIS3 were down-regulated in MSCs from clotted V-BMA in comparison to concentrated one. The study demonstrated for the first time that the cellular source inside the clotted V-BMA showed the best biological properties, representing an alternative and advanced cell therapy approach for patients undergoing spinal surgery.

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MeSH Term

Adipogenesis
Antigens, Surface
Bone Marrow Cells
Cell Culture Techniques
Cell Lineage
Cell Separation
Chondrogenesis
Female
Humans
Middle Aged
Osteogenesis
Spine
Surgical Wound
Transcriptome
Wound Healing

Chemicals

Antigens, Surface
Comparative Study Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 2

Word Cloud

Created with Highcharts 10.0.0V-BMAMSCsclottedspinalcellVertebralsitetherapyapproachsurgerystudybiologicalshowedcellularabilitydifferentiatecomparisonbodybonemarrowaspirateeasilyaccessiblesimultaneouslypreparationpediclescrewinsertionproceduresbecomingincreasinglyusedHowevermaindrawbacksuselackstandardizedprocedurestructuraltexturepossibilitydiffusionawayimplantaimevaluatecharacterizecomparecharacteristicswholeconcentrateV-BMAshighestviabilitygrowthfactorsexpressionTGF-βVEGF-AFGF2greatestcolonyformingunitCFUpotencyhomogeneitytowardsosteogenicCOL1AITNFRSF11BBGLAPchondrogenicphenotypeSOX9lowesttowardadipogeniclineageADIPOQcultureconditionsAdditionallyresultsrevealeddifferentlyisolatedexpresseddifferentlevelHOXTALEsignaturesPBX1MEIS3down-regulatedconcentratedonedemonstratedfirsttimesourceinsidebestpropertiesrepresentingalternativeadvancedpatientsundergoingRationaleUseClottedBoneMarrowAidTissueRegenerationFollowingSpinalSurgery

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