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Applied microbiology and biotechnology
May, 2020;104 (9): 4081-4092.

Gold nanoparticles biosynthesized by Nocardiopsis dassonvillei NCIM 5124 enhance osteogenesis in gingival mesenchymal stem cells.

Tahsin Bennur, Vaishali Javdekar, Geetanjali B Tomar, Smita Zinjarde
Author Information
  1. Tahsin Bennur: Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India.
  2. Vaishali Javdekar: Department of Biotechnology, Abasaheb Garware College, Pune, Maharashtra, 411004, India.
  3. Geetanjali B Tomar: Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India. geetanjalitomar13@gmail.com.
  4. Smita Zinjarde: Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India. smita@unipune.ac.in.
PMID: 32157422 DOI: 10.1007/s00253-020-10508-z

Abstract

Gold nanoparticles are widely used for biomedical applications owing to their biocompatibility, ease of functionalization and relatively non-toxic nature. In recent years, biogenic nanoparticles have gained attention as an eco-friendly alternative for a variety of applications. In this report, we have synthesized and characterized Gold nanoparticles (AuNPs) from an Actinomycete, Nocardiopsis dassonvillei NCIM 5124. The conditions for biosynthesis were optimized (100 mg/ml of cell biomass, 2.5 mM tetrachloroauric acid (HAuCl) at 80 °C and incubation time of 25 min) and the nanoparticles were characterized by TEM, SAED, EDS and XRD analysis. The nanoparticles were spherical and ranged in size from 10 to 25 nm. Their interactions with human gingival tissue-derived mesenchymal stem cells (GMSCs) and their potential applications in regenerative medicine were evaluated further. The AuNPs did not display cytotoxicity towards GMSCs when assessed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay, DNA fragmentation patterns and Annexin V/propidium iodide staining techniques. These AuNPs induced faster cell migration when monitored by the in vitro wound healing assay. The effect of these nanoparticles on osteogenesis of GMSCs was also studied. Based on the results obtained from alkaline phosphatase, Von Kossa staining and Alizarin Red S staining, the AuNPs were seen to positively affect differentiation of GMSCs and enhance mineralization of the synthesized matrix. We therefore conclude that the biogenic, non-toxic AuNPs are of potential relevance for tissue regeneration applications.

Keywords

Biosynthesis Gingiva Gold nanoparticles Mesenchymal stem cells Nocardiopsis dassonvillei NCIM 5124 Osteogenesis

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Grants

  1. UPE Phase II/University Grants Commission
  2. CSIR JRF-SRF/Council of Scientific and Industrial Research
  3. IFA13 LSBM73/Department of Science and Technology INSPIRE, Govt. of India

MeSH Term

Adult
Cell Differentiation
Cell Proliferation
Cells, Cultured
Gingiva
Gold
Humans
Mesenchymal Stem Cells
Metal Nanoparticles
Middle Aged
Nocardiopsis
Osteogenesis

Chemicals

Gold
Journal Article

Word Cloud

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