Innocenzo Muzzalupo, Giuliana Badolati, Adriana Chiappetta, Nevio Picci, Rita Muzzalupo
Olive leaf extract is characterized by a high content of phenols and flavonoids (oleuropein, luteolin, and their derivatives). These compounds are defined as secondary metabolites and exert such as anti-inflammatory, antioxidant, and antimicrobial activities. We investigated the antifungal activity of two Olive leaf extracts (named and ) against a (AACC0215) strain that causes diseases to many economically important plants and synthesizing diverse mycotoxins. In this work, we aimed to identify the most appropriate concentration between the tested two Olive leaf extracts to develop a safe, stable and efficient drug delivery system. Qualitative and quantitative analyses of the two Olive leaf extracts by (HPLC) were performed. Furthermore, we also evaluated the antifungal effects of the two leaf extracts when encapsulated in Chitosan-tripolyphosphate nanoparticles. The major compound in both and was oleuropein, with 336 and 603 mg/g, respectively, however, high concentrations of flavonoid were also present. and showed a concentration depended effect on (AACC0215) viability. Our results showed a great efficacy of /nanoparticles at the higher concentration tested (12X) against the target species. In this case, we observed an inhibition rate to both germination and growth of 87.96 and 58.13%, respectively. We suggest that Olive leaf extracts, as free or encapsulated in Chitosan-tripolyphosphate nanoparticles, could be used as fungicides to control plant diseases. Finally, future application of these findings may allow to reduce the dosage of fungicides potentially harmful to human health.
