The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka.
Mayuri Napagoda, Jana Gerstmeier, Hannah Butschek, Sudhara De Soyza, Simona Pace, Sybille Lorenz, Mallique Qader, Sanjeeva Witharana, Ajith Nagahawatte, Gaya Wijayaratne, Aleš Svatoš, Lalith Jayasinghe, Andreas Koeberle, Oliver Werz
Author Information
Mayuri Napagoda: Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka.
Jana Gerstmeier: Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany.
Hannah Butschek: Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany.
Sudhara De Soyza: Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka.
Simona Pace: Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany.
Sybille Lorenz: Research Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.
Mallique Qader: National Institute of Fundamental Studies, Kandy 20000, Sri Lanka.
Sanjeeva Witharana: Faculty of Engineering, Higher Colleges of Technology, PO Box 4793 Abu Dhabi, UAE.
Ajith Nagahawatte: Department of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka.
Gaya Wijayaratne: Department of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka. ORCID
Aleš Svatoš: Research Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.
Lalith Jayasinghe: National Institute of Fundamental Studies, Kandy 20000, Sri Lanka.
Andreas Koeberle: Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany. ORCID
Oliver Werz: Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany.
Traditional folk medicine in Sri Lanka is mostly based on plants and plant-derived products, however, many of these medicinal plant species are scientifically unexplored. Here, we evaluated the anti-inflammatory and antimicrobial potency of 28 different extracts prepared from seven popular medicinal plant species employed in Sri Lanka. The extracts were subjected to cell-based and cell-free assays of 5-lipoxygenase (5-LO), microsomal prostaglandin E synthase (mPGES)-1, and nitric oxide (NO) scavenging activity. Moreover, antibacterial and disinfectant activities were assessed. Characterization of secondary metabolites was achieved by gas chromatography coupled to mass spectrometric (GC-MS) analysis. -Hexane- and dichloromethane-based extracts of efficiently suppressed 5-LO activity in human neutrophils (IC = 0.92 and 1.39 µg/mL), and potently inhibited isolated human5-LO (IC = 0.15 and 0.16 µg/mL) and mPGES-1 (IC = 0.29 and 0.49 µg/mL). Lipophilic extracts of displayed potent inhibition of mPGES-1 only. A methanolic extract of caused significant NO scavenging activity. The lipophilic extracts of exhibited prominent antibacterial and disinfectant activities, and GC-MS analysis revealed the presence of fatty acids, sesquiterpenes and other types of secondary metabolites. Together, our results suggest the prospective utilization of as disinfective agent with potent anti-inflammatory properties.
