Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of and Regulates IAA Biosynthesis by Interacting LetrpE. - National Genomics Data Center (CNCB-NGDC)

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Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of and Regulates IAA Biosynthesis by Interacting LetrpE.

Gangzheng Wang, Yi Luo, Chen Wang, Yan Zhou, Chunye Mou, Heng Kang, Yang Xiao, Yinbing Bian, Yu Hua Gong

Author Information

Gangzheng Wang: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Yi Luo: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Chen Wang: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Yan Zhou: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Chunye Mou: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Heng Kang: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Yang Xiao: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Yinbing Bian: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Yu Hua Gong: Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

PMID: 32362887 DOI: 10.3389/fmicb.2020.00707

Our previous study found that RNAi decreased resistance to heat stress and infection. In this study, the structure and function of the gene was analyzed by gene cloning and overexpression in stress-sensitive strain YS55 via the -mediated transformation method. Transformants were confirmed by qRT-PCR, fluorescence observation and Southern blotting. Overexpression of in YS55 not only enhanced mycelial resistance to heat stress but also facilitated mycelial growth. In the presence of heat stress, the intracellular IAA content showed a significant increase in the two overexpression strains but only a slight change in the YS55 wild type strain. Moreover, the interaction between LeDnaJ07 and LetrpE was demonstrated via Y2H and BiFC assays. These results suggested that LeDnaJ07 may be involved in regulating IAA biosynthesis and the resistance of to heat stresses via interacting with LetrpE.

BiFC DnaJ protein IAA Lentinula edodes LetrpE thermotolerance yeast two-hybrid

Gene. 2015 Dec 10;574(1):11-9 [PMID: 26210810]
J Exp Bot. 2014 Jan;65(1):143-58 [PMID: 24227338]
Microbiologyopen. 2016 Aug;5(4):709-18 [PMID: 27147196]
Genes (Basel). 2017 Nov 21;8(11): [PMID: 29160795]
PLoS One. 2014 Mar 10;9(3):e91298 [PMID: 24614118]
J Biol Chem. 1992 Sep 15;267(26):18890-5 [PMID: 1527016]
Biotechnol Lett. 2012 Oct;34(10):1915-9 [PMID: 22763851]
PLoS One. 2016 Aug 08;11(8):e0160336 [PMID: 27500531]
Int J Mol Sci. 2019 Jan 16;20(2): [PMID: 30654548]
Appl Microbiol Biotechnol. 2015 Apr;99(7):3115-26 [PMID: 25573474]
PLoS One. 2017 Oct 26;12(10):e0187055 [PMID: 29073211]
Turk J Biol. 2018 Feb 15;42(1):12-22 [PMID: 30814866]
J Exp Bot. 2015 Jun;66(11):3027-40 [PMID: 25801077]
Plant Physiol. 2005 Sep;139(1):151-62 [PMID: 16113208]
Plant Cell. 2011 Feb;23(2):499-514 [PMID: 21343416]
Microb Cell. 2017 Oct 02;4(10):342-361 [PMID: 29082232]
Plant Physiol. 2011 Mar;155(3):1312-24 [PMID: 21209278]
Fungal Genet Biol. 2018 Sep;118:37-44 [PMID: 30003956]
Appl Environ Microbiol. 2018 May 1;84(10): [PMID: 29572207]
Environ Microbiol. 2017 Apr;19(4):1653-1668 [PMID: 28198137]
Plant Sci. 2018 Jul;272:62-74 [PMID: 29807607]
New Phytol. 2012 Apr;194(2):364-78 [PMID: 22356282]
Planta. 2013 Jun;237(6):1509-25 [PMID: 23494613]
Microbiol Res. 2011 May 20;166(4):314-22 [PMID: 20869218]
Mol Plant Pathol. 2018 Mar;19(3):607-614 [PMID: 28220688]
Fungal Genet Biol. 2012 Jan;49(1):15-20 [PMID: 22202810]
J Integr Plant Biol. 2014 Jan;56(1):63-74 [PMID: 24148796]
Curr Top Med Chem. 2012;12(22):2479-90 [PMID: 23339302]
Front Plant Sci. 2016 Mar 24;7:360 [PMID: 27047527]
Cell Physiol Biochem. 2018;50(5):1617-1637 [PMID: 30384356]
New Phytol. 2018 Jan;217(2):739-755 [PMID: 29076546]

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