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06 01, 2020;3 (1): 275.

Integrative omics analysis of the termite gut system adaptation to Miscanthus diet identifies lignocellulose degradation enzymes.

Magdalena Calusinska, Martyna Marynowska, Marie Bertucci, Boris Untereiner, Dominika Klimek, Xavier Goux, David Sillam-Dussès, Piotr Gawron, Rashi Halder, Paul Wilmes, Pau Ferrer, Patrick Gerin, Yves Roisin, Philippe Delfosse
Author Information
  1. Magdalena Calusinska: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg. magdalena.calusinska@list.lu. ORCID
  2. Martyna Marynowska: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg.
  3. Marie Bertucci: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg.
  4. Boris Untereiner: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg.
  5. Dominika Klimek: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg.
  6. Xavier Goux: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg.
  7. David Sillam-Dussès: Laboratory of Experimental and Comparative Ethology EA444, University Paris 13-Sorbonne Paris Cité, 399 Avenue Jean-Baptiste Clément, 93430, Villetaneuse, France.
  8. Piotr Gawron: Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 7 Avenue des Hauts-Fourneaux, L-4362, Esch-sur-Alzette, Luxembourg.
  9. Rashi Halder: Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 7 Avenue des Hauts-Fourneaux, L-4362, Esch-sur-Alzette, Luxembourg. ORCID
  10. Paul Wilmes: Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 7 Avenue des Hauts-Fourneaux, L-4362, Esch-sur-Alzette, Luxembourg.
  11. Pau Ferrer: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg. ORCID
  12. Patrick Gerin: Laboratory of Bioengineering, Earth and Life Institute, Université Catholique de Louvain, Croix du Sud 2/L7.05.19, 1348, Louvain-la-Neuve, Belgium. ORCID
  13. Yves Roisin: Evolutionary Biology and Ecology, Université Libre de Bruxelles, Avenue F.D. Roosevelt 50, 1050, Brussels, Belgium.
  14. Philippe Delfosse: BioSystems and Bioprocessing Engineering, Luxembourg Institute of Science and Technology, Rue du Brill 41, L-4422, Belvaux, Luxembourg.
PMID: 32483294 DOI: 10.1038/s42003-020-1004-3

Abstract

Miscanthus sp. biomass could satisfy future biorefinery value chains. However, its use is largely untapped due to high recalcitrance. The termite and its gut microbiome are considered the most efficient lignocellulose degrading system in nature. Here, we investigate at holobiont level the dynamic adaptation of Cortaritermes sp. to imposed Miscanthus diet, with a long-term objective of overcoming lignocellulose recalcitrance. We use an integrative omics approach combined with enzymatic characterisation of carbohydrate active enzymes from termite gut Fibrobacteres and Spirochaetae. Modified gene expression profiles of gut bacteria suggest a shift towards utilisation of cellulose and arabinoxylan, two main components of Miscanthus lignocellulose. Low identity of reconstructed microbial genomes to closely related species supports the hypothesis of a strong phylogenetic relationship between host and its gut microbiome. This study provides a framework for better understanding the complex lignocellulose degradation by the higher termite gut system and paves a road towards its future bioprospecting.

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MeSH Term

Adaptation, Biological
Animals
Bacteria
Diet
Digestion
Gastrointestinal Microbiome
Gastrointestinal Tract
Gene Expression
Isoptera
Poaceae
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 9

Word Cloud

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