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Methods in molecular biology (Clifton, N.J.)
2020;2145 1-15.

Isolation and Culture of Corneal Stromal Stem Cells.

Richard M Nagymihaly, Morten C Moe, Goran Petrovski
Author Information
  1. Richard M Nagymihaly: Department of Ophthalmology, Center for Eye Research, Oslo University Hospital, Oslo, Norway.
  2. Morten C Moe: Department of Ophthalmology, Center for Eye Research, Oslo University Hospital, Oslo, Norway.
  3. Goran Petrovski: Department of Ophthalmology, Center for Eye Research, Oslo University Hospital, Oslo, Norway. goran.petrovski@medisin.uio.no.
PMID: 32542596 DOI: 10.1007/978-1-0716-0599-8_1

Abstract

An increasing body of evidence authenticates the benefit of corneal stroma-derived stem cells (CSSCs) in tissue engineering and regeneration oriented research, and potentially in the development of clinically relevant cellular therapies. Postmortem corneal tissue obtained from otherwise discarded material after keratoplasties is oftentimes the source of the cells for ex vivo research. Relatively easy to isolate and cultivate as well as inexpensive to culture, CSSCs now represent a well-described cell type with attributes of mesenchymal stem cells (MSCs). These include differentiation- and immunosuppressive potential, as well as a favorable capacity to expand in vitro. Here, we in detail describe two straightforward methods to isolate and establish CSSC cultures ex vivo.

Keywords

Cell culturing Cornea Corneal fibroblasts Corneal stroma Corneal stromal stem cells Isolation Keratocytes Mesenchymal stem cells

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MeSH Term

Cell Culture Techniques
Cell Differentiation
Cornea
Corneal Keratocytes
Corneal Stroma
Corneal Transplantation
Extracellular Matrix
Humans
Mesenchymal Stem Cells
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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