Reduction of 5-fluorouracil-induced toxicity by Sarcodon aspratus polysaccharides in Lewis tumor-bearing mice.
Jiao-Jiao Liu, Juan Chen, Ya Wang, Chen-Chen Yan, Chan Zhang, Shomaila Mehmood, Wen-Juan Pan, Wen-Na Zhang, Yong-Ming Lu, Qing-Xi Wu, Lei Chen, Yan Chen
Author Information
Jiao-Jiao Liu: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
Juan Chen: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
Ya Wang: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
Chen-Chen Yan: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
Chan Zhang: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
Shomaila Mehmood: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
Wen-Juan Pan: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei 230601, Anhui, China; Anhui Key Laboratory of Eco-engineering and Biotechnology, Hefei 230601, Anhui, China.
Wen-Na Zhang: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei 230601, Anhui, China; Anhui Key Laboratory of Eco-engineering and Biotechnology, Hefei 230601, Anhui, China.
Yong-Ming Lu: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei 230601, Anhui, China; Anhui Key Laboratory of Eco-engineering and Biotechnology, Hefei 230601, Anhui, China.
Qing-Xi Wu: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei 230601, Anhui, China; Anhui Key Laboratory of Eco-engineering and Biotechnology, Hefei 230601, Anhui, China.
Lei Chen: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei 230601, Anhui, China; Anhui Key Laboratory of Eco-engineering and Biotechnology, Hefei 230601, Anhui, China.
Yan Chen: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei 230601, Anhui, China; Anhui Key Laboratory of Eco-engineering and Biotechnology, Hefei 230601, Anhui, China. Electronic address: chenyan91030@yahoo.com.
5-Fluorouracil (5-Fu) is an effective anticarcinogenic agent, however, continuous use of 5-Fu may cause severe side effects. The goal of this study was to investigate the effectiveness of Sarcodon aspratus polysaccharides (SATP) in alleviating 5-Fu-induced toxicity in Lewis tumor-bearing mice. Lewis tumor-bearing mice were treated with saline, SATP, 5-Fu or 5-Fu + SATP. The results indicated that compared to the 5-Fu group, the 5-Fu + SATP group showed effective amelioration of the liver, kidney and small intestine injury caused by 5-Fu and decreases in the levels of related biochemical indicators, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT) and urea nitrogen (BUN). Additionally, the combination therapy enhanced the quality of life and immune organ indexes of mice. Further mechanistic studies indicated that the 5-Fu + SATP group showed a decrease in hepatotoxicity caused by 5-Fu via a reduction in the levels of interleukin-1β (IL-1β), an increase in the expression of Bcl-2 and decreases in the expression of p-p38, p-JNK and Bax. Collectively, the results indicated that SATP could significantly alleviate the toxicity of 5-Fu in Lewis tumor-bearing mice and showed the hepatoprotective capability of SATP via its effect on the expression levels of inflammatory factors and components of the MAPK/P38/JNK pathway, which shows that it may be a potential adjuvant for the chemotherapeutic drug 5-Fu in cancer treatment.
