The Absorption Characteristics of Nonvolatile Components in a Water Extraction From as Determined by Single-Pass Intestinal Perfusion and High-Performance Liquid Chromatography.
Yuebao Yao: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Wenjuan Mi: Institute of Chinese Medicine Research, Yangtze River Pharmaceutical Group Jiangsu Longfeng Tang Traditional Chinese Medicine Co., Ltd., Taizhou, China.
Guangzhao Cao: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Ruiqi Yang: Chinese Medicine Research Center, Beijing Increase Innovative Medicine Co., Ltd, Beijing, China.
Huirong Chen: Department of Clinical Study, Beijing Highthink Pharmaceutical Technology Service Co., Ltd, Beijing, China.
Yong Liu: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Huiqin Zou: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Yonghong Yan: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
BACKGROUND: is a famous traditional Chinese medicine (TCM) that can exert beneficial effects during the treatment of gastrointestinal diseases and is used widely in China and other countries in Southeast Asia. However, the nonvolatile active ingredients that are present in the water extractions from used to treat gastrointestinal diseases have yet to be elucidated. The goal of this study was to identify the nonvolatile active ingredients of . METHODS: We used an single-pass intestinal perfusion (SPIP) model to identify the active ingredients of that play significant roles in gastrointestinal absorption. In addition, we developed a high-performance liquid chromatography (HPLC) method to identify key fractions in intestinal outflow perfusate. RESULTS: Nineteen components were identified in a water extraction from ; these exhibited different absorption capabilities in different intestinal segments. Of these, six components were determined by the newly developed HPLC method: catechin, vanillic acid, epicatechin, polydatin, isoquercitrin, and quercitrin. CONCLUSIONS: The current study aimed to identify the active ingredients present in water extractions prepared from in a single-intestinal perfusate from rats. Our findings provide an experimental basis to explain the pharmacodynamic actions of .
