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06 26, 2020;10 (1): 10453.

Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress.

Kamonwan Chamchoy, Pornpan Pumirat, Onrapak Reamtong, Danaya Pakotiprapha, Ubolsree Leartsakulpanich, Usa Boonyuen
Author Information
  1. Kamonwan Chamchoy: Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
  2. Pornpan Pumirat: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
  3. Onrapak Reamtong: Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
  4. Danaya Pakotiprapha: Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
  5. Ubolsree Leartsakulpanich: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand.
  6. Usa Boonyuen: Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand. usa.boo@mahidol.ac.th.
PMID: 32591552 DOI: 10.1038/s41598-020-67382-y

Abstract

A bpss2242 gene, encoding a putative short-chain dehydrogenase/oxidoreductase (SDR) in Burkholderia pseudomallei, was identified and its expression was up-regulated by ten-fold when B. pseudomallei was cultured under high salt concentration. Previous study suggested that BPSS2242 plays important roles in adaptation to salt stress and pathogenesis; however, its biological functions are still unknown. Herein, we report the biochemical properties and functional characterization of BPSS2242 from B. pseudomallei. BPSS2242 exhibited NADPH-dependent reductase activity toward diacetyl and methylglyoxal, toxic electrophilic dicarbonyls. The conserved catalytic triad was identified and found to play critical roles in catalysis and cofactor binding. Tyr162 and Lys166 are involved in NADPH binding and mutation of Lys166 causes a conformational change, altering protein structure. Overexpression of BPSS2242 in Escherichia coli increased bacterial survival upon exposure to diacetyl and methylglyoxal. Importantly, the viability of B. pseudomallei encountered dicarbonyl toxicity was enhanced when cultured under high salt concentration as a result of BPSS2242 overexpression. This is the first study demonstrating that BPSS2242 is responsible for detoxification of toxic metabolites, constituting a protective system against reactive carbonyl compounds in B. pseudomallei..

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MeSH Term

Bacterial Proteins
Burkholderia pseudomallei
NADP
Oxidoreductases
Salt Stress
Sequence Alignment
Sequence Analysis, DNA
Short Chain Dehydrogenase-Reductases

Chemicals

Bacterial Proteins
NADP
Oxidoreductases
Short Chain Dehydrogenase-Reductases
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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