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08 13, 2020;10 (1): 13758.

Molecular fluorescence spectroscopy with multi-way analysis techniques detects spectral variations distinguishing uninfected serum versus dengue or chikungunya viral infected samples.

Marfran C D Santos, Joelma D Monteiro, Josélio M G Araújo, Kássio M G Lima
Author Information
  1. Marfran C D Santos: Biological Chemistry and Chemometrics, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, 59072-970, Brazil.
  2. Joelma D Monteiro: Laboratory of Molecular Biology for Infectious Diseases and Cancer, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, 59072-970, Natal, Brazil.
  3. Josélio M G Araújo: Laboratory of Molecular Biology for Infectious Diseases and Cancer, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, 59072-970, Natal, Brazil.
  4. Kássio M G Lima: Biological Chemistry and Chemometrics, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, 59072-970, Brazil. kassiolima@gmail.com.
PMID: 32792638 DOI: 10.1038/s41598-020-70811-7

Abstract

Significant attempts are being made worldwide in an attempt to develop a tool that, with a simple analysis, is capable of distinguishing between different arboviruses. Herein, we employ molecular fluorescence spectroscopy as a sensitive and specific rapid tool, with simple methodology response, capable of identifying spectral variations between serum samples with or without the dengue or chikungunya viruses. Towards this, excitation emission matrices (EEM) of clinical samples from patients with dengue or chikungunya, in addition to uninfected controls, were separated into a training or test set and analysed using multi-way classification models such as n-PLSDA, PARAFAC-LDA and PARAFAC-QDA. Results were evaluated based on calculations of accuracy, sensitivity, specificity and F score; the most efficient model was identified to be PARAFAC-QDA, whereby 100% was obtained for all figures of merit. QDA was able to predict all samples in the test set based on the scores present in the factors selected by PARAFAC. The loadings obtained by PARAFAC can be used in future studies to prove the direct or indirect relationship of spectral changes caused by the presence of these viruses. This study demonstrates that molecular fluorescence spectroscopy has a greater capacity to detect spectral variations related to the presence of such viruses when compared to more conventional techniques.

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MeSH Term

Algorithms
Chikungunya Fever
Chikungunya virus
Computational Biology
Dengue
Dengue Virus
Humans
Least-Squares Analysis
Molecular Diagnostic Techniques
Principal Component Analysis
Sensitivity and Specificity
Serum
Spectrometry, Fluorescence
Viremia
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

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