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2020;2020 3964518.

Treatment with Hyaluronic Acid and Collagen-Polyvinylpyrrolidone Improves Extracellular Matrix Assembly for Scarring after Tracheal Resection.

J Raúl Olmos-Zuñiga, Matilde Baltazares-Lipp, Claudia Hernández-Jiménez, Rogelio Jasso-Victoria, Miguel Gaxiola-Gaxiola, Mariana Silva-Martínez, Marco Antonio Iñiguez-García, A Ivan González-González, Juan Carlos Vázquez-Minero, Antonia Luna-Flores, Norma Solis-Alanis, Mario Enrique Baltazares-Lipp
Author Information
  1. J Raúl Olmos-Zuñiga: Lung Transplantation Research Unit, Department of Surgical Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico. ORCID
  2. Matilde Baltazares-Lipp: Department of Surgical Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  3. Claudia Hernández-Jiménez: Department of Surgical Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  4. Rogelio Jasso-Victoria: Department of Surgical Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  5. Miguel Gaxiola-Gaxiola: Laboratory of Morphology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  6. Mariana Silva-Martínez: Lung Transplantation Research Unit, Department of Surgical Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  7. Marco Antonio Iñiguez-García: Subdirection of Surgery, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  8. A Ivan González-González: Department of Otolaryngology-Head and Neck Surgery, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  9. Juan Carlos Vázquez-Minero: Subdirection of Surgery, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  10. Antonia Luna-Flores: Lung Transplantation Research Unit, Department of Surgical Research, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
  11. Norma Solis-Alanis: Department of Surgery, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  12. Mario Enrique Baltazares-Lipp: External Consultation Service, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.
PMID: 32908887 DOI: 10.1155/2020/3964518

Abstract

Treatment of tracheal stenosis is occasionally performed in combination with wound healing modulators to manipulate new extracellular matrix (ECM) formation and prevent fibrosis. Hyaluronic acid (HA) and collagen-polyvinylpyrrolidone (collagen-PVP) decrease fibrosis in experimental tracheal healing. However, they have not been used clinically as their effect on ECM components, which modify tracheal scarring, has not been described. . To evaluate the effect of the application of HA, collagen-PVP, a mixture of HA and collagen-PVP (HA+collagen-PVP), and mitomycin C on the expression of decorin, matrix metalloproteinase 1 (MMP1), and MMP9, as well as the type of collagen and deposits formed in the scar after resection and end-to-end anastomosis (REEA) of the cervical trachea using an experimental model. . Thirty dogs underwent REEA of the cervical trachea and were treated with different wound healing modulators: group I ( = 6), control; group II ( = 6), HA; group III ( = 6), collagen-PVP; group IV ( = 6), HA+collagen-PVP; and group V ( = 6), mitomycin C. The dogs were evaluated clinically and endoscopically for 4 weeks. Subsequently, macroscopic and microscopic changes, expression of ECM proteins, and collagen deposition in tracheal scars were analysed. . Groups II, III, and IV showed reduced endoscopic, macroscopic, and microscopic inflammation, improved neovascularization, high decorin expression ( < 0.01, analysis of variance (ANOVA)), and moderate expression of MMP1 ( < 0.003, ANOVA) and type I and III collagen ( < 0.05, Kruskal-Wallis). Groups IV and V developed fewer collagen deposits ( < 0.001, ANOVA). . Treatment with HA and collagen-PVP improved post-REEA healing by increasing neovascularization, stimulating the expression of decorin, and regulating the expression of MMP1, as well as type I and III collagen and their deposition.

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MeSH Term

Anastomosis, Surgical
Animals
Cicatrix
Collagen
Decorin
Disease Models, Animal
Dogs
Extracellular Matrix
Female
Fibrosis
Humans
Hyaluronic Acid
Male
Matrix Metalloproteinase 1
Matrix Metalloproteinase 9
Mitomycin
Postoperative Complications
Povidone
Trachea
Tracheal Stenosis
Wound Healing

Chemicals

Decorin
collagen-PVP
Mitomycin
Hyaluronic Acid
Collagen
Matrix Metalloproteinase 9
Matrix Metalloproteinase 1
Povidone
Journal Article

Word Cloud

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