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Journal of molecular neuroscience : MN
Apr, 2021;71 (4): 869-878.

Severe Zinc Deficiency Causes the Loss and Apoptosis of Olfactory Ensheathing Cells (OECs) and Olfactory Deficit.

Yu Jiang, Lingqi Gu, Zilin Zhang, Jianya Zhao, Chunhua Wan
Author Information
  1. Yu Jiang: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, No. 9, Seyuan Road, Nantong, 226001, People's Republic of China.
  2. Lingqi Gu: Department of Pharmacy, Nantong Maternal and Child Health Hospital, 399 Century Avenue, Nantong, 226018, Jiangsu, China.
  3. Zilin Zhang: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, No. 9, Seyuan Road, Nantong, 226001, People's Republic of China.
  4. Jianya Zhao: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, No. 9, Seyuan Road, Nantong, 226001, People's Republic of China.
  5. Chunhua Wan: Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, No. 9, Seyuan Road, Nantong, 226001, People's Republic of China. Chwan@ntu.edu.cn. ORCID
PMID: 32940875 DOI: 10.1007/s12031-020-01709-2

Abstract

Dietary zinc deficiency may lead to olfactory deficits, whose mechanism remains largely elusive. Olfactory ensheathing cells (OECs), a type of glial cells that support the function and neurogenesis in the olfactory bulb (OB), may play a pivotal role in the maintenance of the olfactory system. In the present study, we established a rat model of dietary zinc deficiency and found that severe zinc deficiency, but not marginal zinc deficiency, caused significantly reduced food intake, growth retardation, and apparent olfactory deficit in growing rats. We showed that severe zinc deficiency resulted in the loss of OECs in the olfactory nerve layer (ONL) of the olfactory bulb. In addition, we revealed that the number of TUNEL-positive cells increased markedly in the region, suggesting an involvement of apoptotic cell death in zinc deficiency-induced loss of OECs. Moreover, we found that treatment with zinc chelator N,N,N'N',-tetrakis (2-pyridylmethyl)ethylenediamine (TPEN) triggered the apoptosis of in vitro-cultured primary OECs. The apoptosis of OECs was correlated with significantly elevated expression of p53. Importantly, TUNEL and CCK-8 assays both demonstrated that treatment with p53 antagonist pifithrin-α (PFT-α) markedly attenuated TPEN-induced OEC apoptosis. These findings implicated that p53-triggered apoptosis of OECs might play an integral role in zinc deficiency-induced olfactory malfunction.

Keywords

Apoptosis Olfactory deficit Olfactory ensheathing cells Zinc deficiency

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Grants

  1. 81300720/Young Scientists Fund

MeSH Term

Animals
Apoptosis
Cells, Cultured
Chelating Agents
Ethylenediamines
Female
Neuroglia
Olfactory Bulb
Rats
Rats, Sprague-Dawley
Smell
Tumor Suppressor Protein p53
Zinc

Chemicals

Chelating Agents
Ethylenediamines
Tumor Suppressor Protein p53
Zinc
N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0zincolfactoryOECsdeficiencyOlfactorycellsapoptosismayensheathingbulbplayrolefoundseveresignificantlydeficitlossmarkedlydeficiency-inducedtreatmentNp53ZincApoptosisDietaryleaddeficitswhosemechanismremainslargelyelusivetypeglialsupportfunctionneurogenesisOBpivotalmaintenancesystempresentstudyestablishedratmodeldietarymarginalcausedreducedfoodintakegrowthretardationapparentgrowingratsshowedresultednervelayerONLadditionrevealednumberTUNEL-positiveincreasedregionsuggestinginvolvementapoptoticcelldeathMoreoverchelatorN'N'-tetrakis2-pyridylmethylethylenediamineTPENtriggeredvitro-culturedprimarycorrelatedelevatedexpressionImportantlyTUNELCCK-8assaysdemonstratedantagonistpifithrin-αPFT-αattenuatedTPEN-inducedOECfindingsimplicatedp53-triggeredmightintegralmalfunctionSevereDeficiencyCausesLossEnsheathingCellsDeficit

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