OpenLB
Open Library of Bioscience
Advanced Search
Biotechnology reports (Amsterdam, Netherlands)
Dec, 2020;28 e00526.

Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from A59 in beta-glucan conversion.

Silvina Ghio, María B Bradanini, Mercedes M Garrido, Ornella M Ontañon, Florencia E Piccinni, Ruben Marrero Diaz de Villegas, Paola M Talia, Eleonora Campos
Author Information
  1. Silvina Ghio: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
  2. María B Bradanini: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
  3. Mercedes M Garrido: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
  4. Ornella M Ontañon: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
  5. Florencia E Piccinni: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
  6. Ruben Marrero Diaz de Villegas: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
  7. Paola M Talia: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
  8. Eleonora Campos: Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina.
PMID: 32963976 DOI: 10.1016/j.btre.2020.e00526

Abstract

In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from A59. Cel8Pa was active on a broad range of substrates, such as β-glucan from barley (24.5 IU/mg), lichenan (17.9 IU/mg), phosphoric acid swollen cellulose (PASC) (9.7 IU/mg), carboxi-methylcellulose (CMC) (7.3 IU/mg), chitosan (1.4 IU/mg) and xylan (0.4 IU/mg). Bg1Pa was active on cellobiose (C2) and cello-oligosaccharides up to C6, releasing glucose as the main product. When both enzymes were used jointly, there was a synergic effect in the conversion rate of polysaccharides to glucose. Cel8Pa and Bg1Pa presented important properties for simultaneous saccharification and fermentation (SSF) processes in second generation bioethanol production, such as tolerance to high concentration of glucose and ethanol.

Keywords

Beta-glucan Beta-glucosidase Endoglucanse GH1 GH8 Paenibacillus Saccharification

References

  1. J Bacteriol. 1992 May;174(9):3087-91 [PMID: 1569036]
  2. Appl Microbiol Biotechnol. 2013 Jul;97(13):5801-13 [PMID: 23076590]
  3. J Comput Chem. 2004 Oct;25(13):1605-12 [PMID: 15264254]
  4. Biotechnol Lett. 2015 Mar;37(3):643-55 [PMID: 25391729]
  5. Nat Methods. 2015 Jan;12(1):7-8 [PMID: 25549265]
  6. Biotechnol Bioeng. 1999;66(1):51-60 [PMID: 10556794]
  7. Biotechnol Biofuels. 2012 Jul 02;5(1):45 [PMID: 22747961]
  8. Proteins. 1998 Dec 1;33(4):567-76 [PMID: 9849940]
  9. Biomacromolecules. 2006 Feb;7(2):644-8 [PMID: 16471942]
  10. Appl Environ Microbiol. 2013 Mar;79(5):1545-54 [PMID: 23263967]
  11. Biosci Biotechnol Biochem. 2014;78(7):1177-82 [PMID: 25229853]
  12. Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5 [PMID: 24270786]
  13. Br Poult Sci. 2018 Oct;59(5):486-493 [PMID: 29877713]
  14. Microbiol Res. 2016 May-Jun;186-187:16-26 [PMID: 27242139]
  15. Appl Environ Microbiol. 2016 Aug 15;82(17):5116-24 [PMID: 27316951]
  16. Biotechnol Lett. 2012 Feb;34(2):281-6 [PMID: 21972145]
  17. Arch Microbiol. 2005 Dec;184(4):215-24 [PMID: 16205911]
  18. Int J Syst Evol Microbiol. 2019 Dec;69(12):3818-3823 [PMID: 31483753]
  19. PLoS One. 2017 Apr 27;12(4):e0176550 [PMID: 28448629]
  20. Genome Announc. 2015 Oct 22;3(5): [PMID: 26494679]
  21. Biotechnol Biofuels. 2016 May 14;9:104 [PMID: 27186238]
  22. Nucleic Acids Res. 2003 Jul 1;31(13):3784-8 [PMID: 12824418]
  23. Appl Microbiol Biotechnol. 2018 May;102(9):3951-3965 [PMID: 29549448]
  24. Structure. 1996 Mar 15;4(3):265-75 [PMID: 8805535]
  25. Nat Biotechnol. 2019 Apr;37(4):420-423 [PMID: 30778233]
  26. Mol Biol Evol. 2013 Dec;30(12):2725-9 [PMID: 24132122]
  27. Food Chem. 2019 Nov 15;298:124999 [PMID: 31261010]
  28. Ann Transl Med. 2014 Feb;2(2):17 [PMID: 25332993]
  29. 3 Biotech. 2016 Jun;6(1):3 [PMID: 28330074]
  30. Trends Biochem Sci. 2016 Jul;41(7):633-645 [PMID: 27211037]
  31. Microb Cell Fact. 2016 Dec 1;15(1):203 [PMID: 27905924]
Journal Article
Article has an altmetric score of 3

Word Cloud

Created with Highcharts 10.0.0IU/mgCel8PaBg1Pa4glucosepolysaccharidesenzymesGH8endoglucanaseGH1β-glucosidaseA59active97conversionefficientbioconversionlignocellulosicbiomassendoglucanasesβ-glucosidaseskeydeconstructionβ-glucansworkfocusedbroadrangesubstratesβ-glucanbarley245lichenan17phosphoricacidswollencellulosePASCcarboxi-methylcelluloseCMC3chitosan1xylan0cellobioseC2cello-oligosaccharidesC6releasingmainproductusedjointlysynergiceffectratepresentedimportantpropertiessimultaneoussaccharificationfermentationSSFprocessessecondgenerationbioethanolproductiontolerancehighconcentrationethanolSynergicactivityβ-1beta-glucanBeta-glucanBeta-glucosidaseEndoglucansePaenibacillusSaccharification

Similar Articles

Cited By