OpenLB
Open Library of Bioscience
Advanced Search
Nature communications
12 16, 2020;11 (1): 6402.

A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge.

Yfat Yahalom-Ronen, Hadas Tamir, Sharon Melamed, Boaz Politi, Ohad Shifman, Hagit Achdout, Einat B Vitner, Ofir Israeli, Elad Milrot, Dana Stein, Inbar Cohen-Gihon, Shlomi Lazar, Hila Gutman, Itai Glinert, Lilach Cherry, Yaron Vagima, Shirley Lazar, Shay Weiss, Amir Ben-Shmuel, Roy Avraham, Reut Puni, Edith Lupu, Elad Bar-David, Assa Sittner, Noam Erez, Ran Zichel, Emanuelle Mamroud, Ohad Mazor, Haim Levy, Orly Laskar, Shmuel Yitzhaki, Shmuel C Shapira, Anat Zvi, Adi Beth-Din, Nir Paran, Tomer Israely
Author Information
  1. Yfat Yahalom-Ronen: Israel Institute for Biological Research, Ness Ziona, Israel.
  2. Hadas Tamir: Israel Institute for Biological Research, Ness Ziona, Israel.
  3. Sharon Melamed: Israel Institute for Biological Research, Ness Ziona, Israel.
  4. Boaz Politi: Israel Institute for Biological Research, Ness Ziona, Israel.
  5. Ohad Shifman: Israel Institute for Biological Research, Ness Ziona, Israel.
  6. Hagit Achdout: Israel Institute for Biological Research, Ness Ziona, Israel.
  7. Einat B Vitner: Israel Institute for Biological Research, Ness Ziona, Israel.
  8. Ofir Israeli: Israel Institute for Biological Research, Ness Ziona, Israel.
  9. Elad Milrot: Israel Institute for Biological Research, Ness Ziona, Israel.
  10. Dana Stein: Israel Institute for Biological Research, Ness Ziona, Israel.
  11. Inbar Cohen-Gihon: Israel Institute for Biological Research, Ness Ziona, Israel.
  12. Shlomi Lazar: Israel Institute for Biological Research, Ness Ziona, Israel.
  13. Hila Gutman: Israel Institute for Biological Research, Ness Ziona, Israel.
  14. Itai Glinert: Israel Institute for Biological Research, Ness Ziona, Israel.
  15. Lilach Cherry: Israel Institute for Biological Research, Ness Ziona, Israel.
  16. Yaron Vagima: Israel Institute for Biological Research, Ness Ziona, Israel.
  17. Shirley Lazar: Israel Institute for Biological Research, Ness Ziona, Israel.
  18. Shay Weiss: Israel Institute for Biological Research, Ness Ziona, Israel.
  19. Amir Ben-Shmuel: Israel Institute for Biological Research, Ness Ziona, Israel.
  20. Roy Avraham: Israel Institute for Biological Research, Ness Ziona, Israel.
  21. Reut Puni: Israel Institute for Biological Research, Ness Ziona, Israel.
  22. Edith Lupu: Israel Institute for Biological Research, Ness Ziona, Israel.
  23. Elad Bar-David: Israel Institute for Biological Research, Ness Ziona, Israel.
  24. Assa Sittner: Israel Institute for Biological Research, Ness Ziona, Israel.
  25. Noam Erez: Israel Institute for Biological Research, Ness Ziona, Israel.
  26. Ran Zichel: Israel Institute for Biological Research, Ness Ziona, Israel.
  27. Emanuelle Mamroud: Israel Institute for Biological Research, Ness Ziona, Israel. ORCID
  28. Ohad Mazor: Israel Institute for Biological Research, Ness Ziona, Israel. ORCID
  29. Haim Levy: Israel Institute for Biological Research, Ness Ziona, Israel. ORCID
  30. Orly Laskar: Israel Institute for Biological Research, Ness Ziona, Israel.
  31. Shmuel Yitzhaki: Israel Institute for Biological Research, Ness Ziona, Israel.
  32. Shmuel C Shapira: Israel Institute for Biological Research, Ness Ziona, Israel.
  33. Anat Zvi: Israel Institute for Biological Research, Ness Ziona, Israel.
  34. Adi Beth-Din: Israel Institute for Biological Research, Ness Ziona, Israel.
  35. Nir Paran: Israel Institute for Biological Research, Ness Ziona, Israel. nirp@iibr.gov.il. ORCID
  36. Tomer Israely: Israel Institute for Biological Research, Ness Ziona, Israel. tomeri@iibr.gov.il. ORCID
PMID: 33328475 DOI: 10.1038/s41467-020-20228-7

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of an efficient and cost-effective vaccine, suitable for mass immunization. Here, we show the development of a replication competent recombinant VSV-∆G-spike vaccine, in which the glycoprotein of VSV is replaced by the spike protein of SARS-CoV-2. In-vitro characterization of this vaccine indicates the expression and presentation of the spike protein on the viral membrane with antigenic similarity to SARS-CoV-2. A golden Syrian hamster in-vivo model for COVID-19 is implemented. We show that a single-dose vaccination results in a rapid and potent induction of SARS-CoV-2 neutralizing antibodies. Importantly, vaccination protects hamsters against SARS-CoV-2 challenge, as demonstrated by the abrogation of body weight loss, and  alleviation of the extensive tissue damage and viral loads in lungs and nasal turbinates. Taken together, we suggest the recombinant VSV-∆G-spike as a safe, efficacious and protective vaccine against SARS-CoV-2.

References

  1. Am J Respir Cell Mol Biol. 2011 May;44(5):725-38 [PMID: 21531958]
  2. Mol Cell. 2020 May 21;78(4):779-784.e5 [PMID: 32362314]
  3. Cell. 2020 Apr 16;181(2):281-292.e6 [PMID: 32155444]
  4. Clin Infect Dis. 2020 Dec 3;71(9):2428-2446 [PMID: 32215622]
  5. Nat Rev Drug Discov. 2020 May;19(5):305-306 [PMID: 32273591]
  6. Nature. 2020 Jul;583(7818):834-838 [PMID: 32408338]
  7. Cell Host Microbe. 2020 Sep 9;28(3):475-485.e5 [PMID: 32735849]
  8. Nat Commun. 2020 Aug 27;11(1):4303 [PMID: 32855401]
  9. J Gen Virol. 2016 Aug;97(8):1853-1864 [PMID: 27145752]
  10. Bioinformatics. 2009 Aug 15;25(16):2078-9 [PMID: 19505943]
  11. Cell. 2020 Apr 16;181(2):271-280.e8 [PMID: 32142651]
  12. Nature. 2020 Mar;579(7798):270-273 [PMID: 32015507]
  13. Sci Rep. 2019 May 23;9(1):7755 [PMID: 31123310]
  14. Microbes Infect. 2020 Oct;22(9):403-404 [PMID: 32599077]
  15. Cell Host Microbe. 2020 Sep 9;28(3):486-496.e6 [PMID: 32738193]
  16. BMJ. 2000 Nov 18;321(7271):1237-8 [PMID: 11082069]
  17. Hum Vaccin Immunother. 2018;14(9):2107-2113 [PMID: 29757706]
  18. Cell Rep. 2020 Mar 24;30(12):3964-3971.e7 [PMID: 32209459]
  19. Proc Natl Acad Sci U S A. 2020 Jul 14;117(28):16587-16595 [PMID: 32571934]
  20. Emerg Microbes Infect. 2020 Dec;9(1):837-842 [PMID: 32301390]
  21. Cell Host Microbe. 2020 Sep 9;28(3):465-474.e4 [PMID: 32798445]
  22. J Gen Virol. 2005 Aug;86(Pt 8):2269-2274 [PMID: 16033974]
  23. Expert Rev Vaccines. 2015;14(11):1509-23 [PMID: 26313239]
  24. Nat Methods. 2012 Mar 04;9(4):357-9 [PMID: 22388286]
  25. Vaccine X. 2019 Jan 29;1:100009 [PMID: 31384731]
  26. Virology. 2005 Sep 30;340(2):174-82 [PMID: 16043204]
  27. Nat Microbiol. 2020 Apr;5(4):536-544 [PMID: 32123347]
  28. EClinicalMedicine. 2020 Sep;26:100525 [PMID: 32923991]
  29. PLoS One. 2014 Oct 28;9(10):e110545 [PMID: 25350003]
  30. Nat Commun. 2020 Mar 27;11(1):1620 [PMID: 32221306]
  31. J Virol Methods. 2010 Nov;169(2):365-74 [PMID: 20709108]
  32. Hum Vaccin Immunother. 2019;15(10):2269-2285 [PMID: 31368826]
  33. Viruses. 2020 Jul 20;12(7): [PMID: 32698441]
  34. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4477-81 [PMID: 7753828]
  35. Chin Med J (Engl). 2020 May 5;133(9):1025-1031 [PMID: 32044814]
  36. Immunity. 2020 Apr 14;52(4):583-589 [PMID: 32259480]
  37. Nature. 2020 Mar;579(7798):265-269 [PMID: 32015508]

MeSH Term

Animals
Antibodies, Viral
Antigens, Viral
Body Weight
COVID-19
COVID-19 Vaccines
Cell Line
Cricetinae
Disease Models, Animal
Dose-Response Relationship, Immunologic
Genome, Viral
Lung
Mice, Inbred C57BL
Mutation
SARS-CoV-2
Spike Glycoprotein, Coronavirus
Vaccination
Vaccines, Synthetic
Vesicular stomatitis Indiana virus
Viral Load

Chemicals

Antibodies, Viral
Antigens, Viral
COVID-19 Vaccines
Spike Glycoprotein, Coronavirus
Vaccines, Synthetic
Journal Article
Article has an altmetric score of 35

Word Cloud

Created with Highcharts 10.0.0SARS-CoV-2vaccinerecombinantVSV-∆G-spikeCOVID-19rapiddevelopmentshowspikeproteinviralvaccinationchallengepandemiccausedimposesurgentneedefficientcost-effectivesuitablemassimmunizationreplicationcompetentglycoproteinVSVreplacedIn-vitrocharacterizationindicatesexpressionpresentationmembraneantigenicsimilaritygoldenSyrianhamsterin-vivomodelimplementedsingle-doseresultspotentinductionneutralizingantibodiesImportantlyprotectshamstersdemonstratedabrogationbodyweightloss and alleviationextensivetissuedamageloadslungsnasalturbinatesTakentogethersuggestsafeefficaciousprotectivesingledoseprovidesprotection

Similar Articles

Cited By