Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line.

Chun Gui, Chao Zhang, Xiaomei Xiong, Jing Huang, Juan Xi, Ling Gong, Bisheng Huang, Xiuqiao Zhang
Author Information
  1. Chun Gui: The Medicinal Plant Garden, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
  2. Chao Zhang: The Medicinal Plant Garden, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
  3. Xiaomei Xiong: The Medicinal Plant Garden, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
  4. Jing Huang: The Medicinal Plant Garden, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
  5. Juan Xi: Department of Clinical Biochemistry, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
  6. Ling Gong: Department of Pharmacognosy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
  7. Bisheng Huang: Department of Pharmacognosy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
  8. Xiuqiao Zhang: Department of Pharmacognosy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.

Abstract

Ampelopsis megalophylla has been found to demonstrate anticancer activities in human cancer cells; however, the effect of total flavone extract (TFE), commonly used in Traditional Chinese Medicine, remains unclear. Furthermore, there is limited information on its effects on breast cancer cell lines. The present study aimed to investigate the inhibitory effects of TFE in different human cancer cell lines. In addition, the underlying mechanisms and the signaling pathways involved were also investigated by determining tumor cell morphological changes, and differences in the cell cycle, apoptosis, mitochondrial transmembrane potential, and related protein expression levels in a breast cancer cell line. It was found that TFE inhibited proliferation in seven cancer cell lines (HeLa, A549, MCF‑7, HepG2, A2780, SW620 and MDA‑MB‑231 and demonstrated a strong inhibitory effect on MCF‑7 cell proliferation. Cell morphological changes were also observed and arrested at the G2/M phase following treatment with TFE at different concentrations. In addition, TFE disrupted the mitochondrial membrane potential and upregulated the expression level of apoptotic proteins, including caspase‑3, ‑8 and ‑9, the Bax/Bcl‑2 ratio, and Apaf‑1 in time‑dependent manner. These results indicated that TFE induced apoptosis of the MCF‑7 cells via a mitochondrial‑mediated apoptotic pathway. In conclusion, TFE is potentially effective in treating breast cancer.

Keywords

References

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MeSH Term

Ampelopsis
Antineoplastic Agents, Phytogenic
Apoptosis
Breast Neoplasms
Female
Humans
MCF-7 Cells
Mitochondria
Plant Extracts

Chemicals

Antineoplastic Agents, Phytogenic
Plant Extracts

Word Cloud

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