The Combination of Jiedu Xiaoluo Decoction with Autologous Peripheral Blood Stem Cell Transplantation (APBSCT) Accelerates Disease Remission of Non-Hodgkin Lymphoma.
Yu Zhang, Jingjing Xiang, Ni Zhu, Hangping Ge, Xianfu Sheng, Shu Deng, Junfa Chen, Lihong Yu, Yan Zhou, Jianping Shen
Author Information
Yu Zhang: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China. ORCID
Jingjing Xiang: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Ni Zhu: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Hangping Ge: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Xianfu Sheng: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Shu Deng: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Junfa Chen: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Lihong Yu: First Medical College of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Yan Zhou: First Medical College of Zhejiang Chinese Medical University, Hangzhou 310000, China.
Jianping Shen: Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, China. ORCID
OBJECTIVE: This study aimed to explore the therapeutic effects of autologous peripheral blood stem cell transplantation (APBSCT) with Jiedu Xiaoluo decoction (JDX) on non-Hodgkin lymphoma (NHL). METHOD: B lymphoma cells A20 were used to establish nude mice-transplanted tumor model. The peripheral blood of mice was analyzed by automatic blood cell counter. Inflammatory cytokines in tumor tissues were measured by ELISA, real-time qRT-PCR, and western blotting assays. Immunohistochemical staining was employed to evaluate tumor cell growth and apoptosis. CCK8 and Transwell assays were used to detect cell viability, migration, and invasion. Cell apoptosis in vitro was evaluated with flow cytometry. RESULT: In the in vitro co-culture system of A20 cells and hemopoietic stem cells (HSC), JDX notably inhibited the proliferation, migration, and invasion and promoted apoptosis of A20 cells compared to HSC treatment alone. In animal tumor xenografts of NHL, the combination of APBSCT with JDX significantly promoted hematopoietic reconstitution, inhibited tumorigenesis of A20 cell, promoted the inflammatory microenvironment remission, inhibited cell proliferation, and promoted apoptosis compared to APBSCT alone. CONCLUSION: The combination of APBSCT with JDX might be an effective strategy to treat NHL through inhibiting tumorigenesis and reconstructing hematopoietic and immune microenvironment. Our finding provided a novel insight into the clinical application of Traditional Chinese Medicine (TCM) against NHL.
