Guiying Yang, Farhan Ahmad, Qihuan Zhou, Meixia Guo, Shiyou Liang, Hassan Ahmed Gaal, Jianchu Mo
species are wild edible mushrooms that possess high nutritional value and a wide range of medicinal properties. However, the cultivation of these mushrooms is very difficult because of their symbiotic association with termites. In this study, we aimed to examine the differences in physicochemical indices and microbial communities between combs with basidiomes (CF) and combs without basidiomes (CNF). High-performance liquid chromatography (HPLC), inductively coupled plasma optical emission spectrometry (ICP-OES), gas chromatography equipped with a flame ionization detector (GC-FID), some commercial kits, high-throughput sequencing of the 16s RNA, and internal transcribed spacer (ITS) were used. Humidity, pH, and elements, i.e., Al, Ba, Fe, Mn, Ni, S, Ca, and Mg were higher while amino acids particularly alanine, tyrosine, and isoleucine were lower in CF as compared to CNF. The average contents of fatty acids were not significantly different between the two comb categories. The bacterial genera , , , and were dominant in all combs. , , and were significantly more abundant in CF. Basidiomycota and Ascomycota were also identified in combs. , sp. Group3, and sp. were the most dominant species in combs. However, any single species was abundantly present in an individual comb.
