On-demand biomanufacturing of protective conjugate vaccines.
Jessica C Stark, Thapakorn Jaroentomeechai, Tyler D Moeller, Jasmine M Hershewe, Katherine F Warfel, Bridget S Moricz, Anthony M Martini, Rachel S Dubner, Karen J Hsu, Taylor C Stevenson, Bradley D Jones, Matthew P DeLisa, Michael C Jewett
Author Information
Jessica C Stark: Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd Technological Institute E136, Evanston, IL 60208-3120, USA. ORCID
Thapakorn Jaroentomeechai: Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853, USA. ORCID
Tyler D Moeller: Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853, USA. ORCID
Jasmine M Hershewe: Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd Technological Institute E136, Evanston, IL 60208-3120, USA. ORCID
Katherine F Warfel: Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd Technological Institute E136, Evanston, IL 60208-3120, USA. ORCID
Bridget S Moricz: Department of Microbiology and Immunology, University of Iowa, 51 Newton Rd 3-403 Bowen Science Building, Iowa City, IA 52242, USA.
Anthony M Martini: Department of Microbiology and Immunology, University of Iowa, 51 Newton Rd 3-403 Bowen Science Building, Iowa City, IA 52242, USA. ORCID
Rachel S Dubner: Department of Biological Sciences, Northwestern University, 2205 Tech Drive Hogan Hall 2144, Evanston, IL 60208-3500, USA. ORCID
Karen J Hsu: Department of Mechanical Engineering, Northwestern University, 2145 Sheridan Rd Technological Institute B224, Evanston, IL 60208-3120, USA.
Taylor C Stevenson: Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Weill Hall, Ithaca, NY 14853, USA.
Bradley D Jones: Department of Microbiology and Immunology, University of Iowa, 51 Newton Rd 3-403 Bowen Science Building, Iowa City, IA 52242, USA. ORCID
Matthew P DeLisa: Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853, USA. md255@cornell.edu m-jewett@northwestern.edu. ORCID
Michael C Jewett: Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd Technological Institute E136, Evanston, IL 60208-3120, USA. md255@cornell.edu m-jewett@northwestern.edu. ORCID
Conjugate vaccines are among the most effective methods for preventing bacterial infections. However, existing manufacturing approaches limit access to conjugate vaccines due to centralized production and cold chain distribution requirements. To address these limitations, we developed a modular technology for in vitro conjugate vaccine expression (iVAX) in portable, freeze-dried lysates from detoxified, nonpathogenic Upon rehydration, iVAX reactions synthesize clinically relevant doses of conjugate vaccines against diverse bacterial pathogens in 1 hour. We show that iVAX-synthesized vaccines against subsp. (type A) strain Schu S4 protected mice from lethal intranasal challenge. The iVAX platform promises to accelerate development of new conjugate vaccines with increased access through refrigeration-independent distribution and portable production.
