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02 03, 2021;10 (2):

Adsorption of Cd to TiO-NPs Forms Low Genotoxic AGGREGATES in Zebrafish Cells.

Filomena Mottola, Marianna Santonastaso, Concetta Iovine, Veronica Feola, Severina Pacifico, Lucia Rocco
Author Information
  1. Filomena Mottola: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
  2. Marianna Santonastaso: Department of Woman, Child and General and Special Surgery, University of Campania "Luigi Vanvitelli", 80138 Napoli, Italy. ORCID
  3. Concetta Iovine: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
  4. Veronica Feola: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
  5. Severina Pacifico: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy. ORCID
  6. Lucia Rocco: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy. ORCID
PMID: 33546308 DOI: 10.3390/cells10020310

Abstract

The aquatic environment is involved in the pollutants spreading mechanisms, including nanomaterials and heavy metals. The aims of this study were to assess the in vivo genotoxicity of Cd (1 mg/L) and to investigate the genomic effects generated by its co-exposure with TiO-NPs (10 µg/L). The study was performed using zebrafish as a model for 5, 7, 14, 21, and 28 days of exposure. The genotoxic potential was assessed by three experimental approaches: DNA integrity, degree of apoptosis, and molecular alterations at the genomic level by genomic template stability (% GTS) calculation. Results showed an increased in DNA damage after Cd exposure with a decrease in % GTS. The co-exposure (TiO-NPs + Cd) induced a no statistically significant loss of DNA integrity, a reduction of the apoptotic cell percentage and the recovery of genome stability for prolonged exposure days. Characterization and analytical determinations data showed Cd adsorption to TiO-NPs, which reduced free TiO-NPs levels. The results of our study suggest that TiO-NPs could be used for the development of controlled heavy metal bioremediation systems.

Keywords

DNA damage apoptotic cells cadmium genome instability titanium dioxide nanoparticles zebrafish

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Grants

  1. POR FESR 2014-2020 ASSE I O.S. 1.2 - Action 1.2.2. (ICURE Project)/Campania Region

MeSH Term

Adsorption
Animals
Cadmium
DNA Damage
Genomic Instability
Nanoparticles
Titanium
Zebrafish

Chemicals

Cadmium
titanium dioxide
Titanium
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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