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02 24, 2021;11 (1): 4492.

Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis).

Yang-Yang Pang, Gen-Yong Huang, Ya-Meng Song, Xiao- Zhe Song, Jia-Huan Lv, Long He, Chao Niu, Ao-Ya Shi, Xing-Liang Shi, Yong-Xu Cheng, Xiao-Zhen Yang
Author Information
  1. Yang-Yang Pang: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  2. Gen-Yong Huang: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  3. Ya-Meng Song: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  4. Xiao- Zhe Song: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  5. Jia-Huan Lv: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  6. Long He: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  7. Chao Niu: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  8. Ao-Ya Shi: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  9. Xing-Liang Shi: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China.
  10. Yong-Xu Cheng: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China. yxcheng@shou.edu.cn.
  11. Xiao-Zhen Yang: National Demonstration Center for Experimental Fisheries Science Education; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Engineering Research Center of Aquaculture, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, People's Republic of China. xzyang@shou.edu.cn.
PMID: 33627750 DOI: 10.1038/s41598-021-83984-6

Abstract

Chinese mitten crab (Eriocheir sinensis) as a commercially important species is widely cultured in China. However, E. sinensis is prone to agonistic behavior, which causes physical damage and wastes energy resources, negatively impacting their growth and survival. Therefore, understanding the regulatory mechanisms that underlie the switching of such behavior is essential for ensuring the efficient and cost-effective aquaculture of E. sinensis. The 5-HT2B receptor is a key downstream target of serotonin (5-HT), which is involved in regulating animal behavior. In this study, the full-length sequence of 5-HT2B gene was cloned. The total length of the 5-HT2B gene was found to be 3127 bp with a 236 bp 5'-UTR (untranslated region), a 779 bp 3'-UTR, and a 2112 bp open reading frame encoding 703 amino acids. Phylogenetic tree analysis revealed that the 5-HT2B amino acid sequence of E. sinensis is highly conserved with that of Cancer borealis. Using in vitro co-culture and luciferase assays, the miR-143 targets the 5-HT2B 3'-UTR and inhibits 5-HT2B expression was confirmed. Furthermore, RT-qPCR and Western blotting analyses revealed that the miR-143 mimic significantly inhibits 5-HT2B mRNA and protein expression. However, injection of miR-143 did not decrease agonistic behavior, indicating that 5-HT2B is not involved in the regulation of such behavior in E. sinensis.

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Grants

  1. CARS-48/China Agriculture Research System
  2. 2019YFD0900105/National Key Research and Development Program of China

MeSH Term

3' Untranslated Regions
5' Untranslated Regions
Agonistic Behavior
Amino Acid Sequence
Animals
Base Sequence
Brachyura
China
Cloning, Molecular
DNA, Complementary
Gene Expression Profiling
MicroRNAs
Open Reading Frames
Phylogeny
RNA, Messenger
Sequence Alignment
Serotonin

Chemicals

3' Untranslated Regions
5' Untranslated Regions
DNA, Complementary
MicroRNAs
RNA, Messenger
Serotonin
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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