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International journal of nanomedicine
2021;16 989-1000.

Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes.

Naim Kittana, Mohyeddin Assali, Wolfram-Hubertus Zimmermann, Norman Liaw, Gabriela Leao Santos, Abdul Rehman, Susanne Lutz
Author Information
  1. Naim Kittana: Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine. ORCID
  2. Mohyeddin Assali: Department of Pharmacy, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine. ORCID
  3. Wolfram-Hubertus Zimmermann: Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany. ORCID
  4. Norman Liaw: Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany. ORCID
  5. Gabriela Leao Santos: Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany. ORCID
  6. Abdul Rehman: Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany.
  7. Susanne Lutz: Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany. ORCID
PMID: 33633447 DOI: 10.2147/IJN.S289107

Abstract

BACKGROUND: Under certain conditions, the physiological repair of connective tissues might fail to restore the original structure and function. Optimized engineered connective tissues (ECTs) with biophysical properties adapted to the target tissue could be used as a substitution therapy. This study aimed to investigate the effect of ECT enforcement by a complex of multiwall carbon nanotubes with chitosan (C-MWCNT) to meet in vivo demands.
MATERIALS AND METHODS: ECTs were constructed from human foreskin fibroblasts (HFF-1) in collagen type I and enriched with the three different percentages 0.025, 0.05 and 0.1% of C-MWCNT. Characterization of the physical properties was performed by biomechanical studies using unidirectional strain.
RESULTS: Supplementation with 0.025% C-MWCNT moderately increased the tissue stiffness, reflected by Young's modulus, compared to tissues without C-MWCNT. Supplementation of ECTs with 0.1% C-MWCNT reduced tissue contraction and increased the elasticity and the extensibility, reflected by the yield point and ultimate strain, respectively. Consequently, the ECTs with 0.1% C-MWCNT showed a higher resilience and toughness as control tissues. Fluorescence tissue imaging demonstrated the longitudinal alignment of all cells independent of the condition.
CONCLUSION: Supplementation with C-MWCNT can enhance the biophysical properties of ECTs, which could be advantageous for applications in connective tissue repair.

Keywords

chitosan collagen-based tissue scaffold engineered connective tissue mechanical properties multiwall carbon nanotubes

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MeSH Term

Animals
Biomechanical Phenomena
Cattle
Cell Line
Chitosan
Connective Tissue
Elastic Modulus
Fibroblasts
Humans
Nanotubes, Carbon
Tissue Engineering

Chemicals

Nanotubes, Carbon
Chitosan
Journal Article
Article has an altmetric score of 2

Word Cloud

Created with Highcharts 10.0.0tissueC-MWCNT0ECTsconnectivetissuesproperties1%SupplementationrepairengineeredbiophysicalmultiwallcarbonnanotubeschitosanstrainincreasedreflectedBACKGROUND:certainconditionsphysiologicalmightfailrestoreoriginalstructurefunctionOptimizedadaptedtargetusedsubstitutiontherapystudyaimedinvestigateeffectECTenforcementcomplexmeetvivodemandsMATERIALSANDMETHODS:constructedhumanforeskinfibroblastsHFF-1collagentypeenrichedthreedifferentpercentages02505CharacterizationphysicalperformedbiomechanicalstudiesusingunidirectionalRESULTS:025%moderatelystiffnessYoung'smoduluscomparedwithoutreducedcontractionelasticityextensibilityyieldpointultimaterespectivelyConsequentlyshowedhigherresiliencetoughnesscontrolFluorescenceimagingdemonstratedlongitudinalalignmentcellsindependentconditionCONCLUSION:canenhanceadvantageousapplicationsModulatingBiomechanicalPropertiesEngineeredConnectiveTissuesChitosan-CoatedMultiwallCarbonNanotubescollagen-basedscaffoldmechanical

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