OpenLB
Open Library of Bioscience
Advanced Search
Antibiotics (Basel, Switzerland)
Feb 28, 2021;10 (3):

Methicillin-Resistant Clonal Complex 398 as a Major MRSA Lineage in Dogs and Cats in Thailand.

Surawit Chueahiran, Jitrapa Yindee, Pongthai Boonkham, Nipattra Suanpairintr, Pattrarat Chanchaithong
Author Information
  1. Surawit Chueahiran: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. ORCID
  2. Jitrapa Yindee: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
  3. Pongthai Boonkham: Veterinary Diagnostic Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
  4. Nipattra Suanpairintr: Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
  5. Pattrarat Chanchaithong: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. ORCID
PMID: 33671008 DOI: 10.3390/antibiotics10030243

Abstract

The aim of this study was to present molecular and antimicrobial resistance characteristics of methicillin-resistant (MRSA) clonal complex (CC) 398 isolated from diseased dogs and cats in Thailand. A total of 20 MRSA isolates of 134 isolated from canine and feline clinical samples during 2017-2020 were CC398, consisting of sequence type (ST) 398 (18 isolates), ST5926 (1 isolate), and ST6563 (1 isolate) by multilocus sequence typing. t034 and staphylococcal cassette chromosome (SCC) V were predominantly associated with ST398. Intraclonal differentiation was present by additional (t1255, t4653), non-detectable , composite SCC with a hybrid of + and class A complex, and DNA fingerprints by pulsed-field gel electrophoresis. The isolates essentially carried antimicrobial resistance genes, mediating multiple resistance to β-lactams (, ), tetracyclines [(M)], aminoglycosides [], and trimethoprim (). Livestock-associated MRSA ST398 resistance genes including (B), (E), , , and (L) were heterogeneously found and lost in subpopulation, with the absence or presence of additional (A), (B), and genes that corresponded to resistance phenotypes. As only a single CC398 was detected with the presence of intraclonal variation, CC398 seems to be the successful MRSA clone colonizing in small animals as a pet-associated MRSA in Thailand.

Keywords

MRSA antimicrobial resistance cat clonal complex 398 dog

References

  1. Vet Rec. 2010 Jun 26;166(26):826-7 [PMID: 20581361]
  2. Vet Microbiol. 2019 Aug;235:118-126 [PMID: 31282369]
  3. Microb Drug Resist. 2018 Mar;24(2):203-212 [PMID: 28598251]
  4. Appl Environ Microbiol. 2013 Jan;79(2):655-62 [PMID: 23160118]
  5. J Med Microbiol. 2007 Jul;56(Pt 7):937-939 [PMID: 17577059]
  6. J Glob Antimicrob Resist. 2020 Mar;20:253-259 [PMID: 31472282]
  7. Antimicrob Agents Chemother. 2007 Jan;51(1):264-74 [PMID: 17043114]
  8. Prev Vet Med. 2016 Apr 1;126:190-8 [PMID: 26948298]
  9. J Clin Microbiol. 2003 Sep;41(9):4089-94 [PMID: 12958230]
  10. Microb Drug Resist. 2019 Nov;25(9):1382-1389 [PMID: 31361580]
  11. J Clin Microbiol. 2003 Dec;41(12):5442-8 [PMID: 14662923]
  12. PLoS One. 2015 Aug 31;10(8):e0137143 [PMID: 26322785]
  13. Int J Med Microbiol. 2014 Oct;304(7):787-93 [PMID: 25130703]
  14. J Antimicrob Chemother. 2011 Jun;66(6):1236-42 [PMID: 21436154]
  15. Sci Rep. 2017 Jul 13;7(1):5273 [PMID: 28706213]
  16. Clin Microbiol Infect. 2012 Aug;18(8):745-55 [PMID: 22509728]
  17. J Clin Microbiol. 2000 Mar;38(3):1008-15 [PMID: 10698988]
  18. Diagn Microbiol Infect Dis. 2001 Jul;40(3):111-6 [PMID: 11502378]
  19. Antimicrob Agents Chemother. 2009 Dec;53(12):4961-7 [PMID: 19721075]
  20. PLoS One. 2014 Jan 20;9(1):e85656 [PMID: 24465637]
  21. Antimicrob Agents Chemother. 2013 Nov;57(11):5509-15 [PMID: 23979735]
  22. Int J Antimicrob Agents. 2012 Apr;39(4):273-82 [PMID: 22230333]
  23. J Clin Microbiol. 2010 Mar;48(3):765-9 [PMID: 20053855]
  24. Infect Control Hosp Epidemiol. 2006 Oct;27(10):1025-31 [PMID: 17006808]
  25. Berl Munch Tierarztl Wochenschr. 2006 May-Jun;119(5-6):222-32 [PMID: 16729469]
  26. Int J Antimicrob Agents. 2015 Apr;45(4):334-40 [PMID: 25593014]
  27. Vet Microbiol. 2015 Jun 12;177(3-4):353-8 [PMID: 25891423]
  28. Clin Infect Dis. 2008 Mar 15;46(6):787-94 [PMID: 18266611]
  29. Genome Biol Evol. 2017 Apr 1;9(4):830-842 [PMID: 28338786]
  30. J Clin Microbiol. 2008 Feb;46(2):574-81 [PMID: 18032612]
  31. Curr Opin Microbiol. 2012 Oct;15(5):588-95 [PMID: 23044073]
  32. mBio. 2012 Feb 21;3(1): [PMID: 22354957]
  33. Clin Infect Dis. 2006 Mar 1;42(5):647-56 [PMID: 16447110]
  34. J Antimicrob Chemother. 2012 Feb;67(2):326-9 [PMID: 22146878]
  35. Vet Microbiol. 2018 Jul;221:153-158 [PMID: 29981702]
  36. mBio. 2014 May 13;5(3):e00985-13 [PMID: 24825010]
  37. J Appl Microbiol. 2014 Aug;117(2):572-86 [PMID: 24833550]
  38. Zoonoses Public Health. 2013 Aug;60(5):366-74 [PMID: 22925210]
  39. J Antimicrob Chemother. 2010 Jun;65(6):1145-54 [PMID: 20348087]
  40. Vet J. 2014 Feb;199(2):217-22 [PMID: 24345778]
  41. Vet Dermatol. 2012 Aug;23(4):276-82, e55 [PMID: 22681522]
  42. Clin Infect Dis. 2003 Jan 15;36(2):e26-8 [PMID: 12522764]
  43. J Antimicrob Chemother. 2013 Jun;68(6):1256-66 [PMID: 23425780]
  44. Lett Appl Microbiol. 2010 Jan;50(1):127-30 [PMID: 19843206]
  45. J Bacteriol. 2005 Nov;187(21):7292-308 [PMID: 16237012]
  46. Vet Dermatol. 2014 Jun;25(3):163-e43 [PMID: 24720433]
  47. J Glob Antimicrob Resist. 2016 Dec;7:126-127 [PMID: 27750159]
  48. Antibiotics (Basel). 2019 Mar 16;8(1): [PMID: 30884836]
  49. BMC Vet Res. 2020 Jan 6;16(1):4 [PMID: 31906922]
  50. Vet Microbiol. 2010 Jan 27;140(3-4):418-29 [PMID: 19246166]
  51. J Antimicrob Chemother. 2013 Jun;68(6):1251-5 [PMID: 23386262]
  52. Vet J. 2011 Nov;190(2):e125-e129 [PMID: 21382731]
  53. Mol Microbiol. 2010 Sep;77(6):1583-94 [PMID: 20860091]
  54. Vet Dermatol. 2012 Aug;23(4):330-41, e62 [PMID: 22731400]
  55. Front Microbiol. 2017 Dec 13;8:2493 [PMID: 29326664]

Grants

  1. 4/2561/Chulalongkorn University-Veterinary Science Research Fund
  2. no grant number/Chulalongkorn Academic Advancement into Its 2nd Century Project
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0MRSAresistance398antimicrobialcomplexThailandisolatesCC398genespresentclonalisolatedsequence1isolateSCCST398additionalBpresenceaimstudymolecularcharacteristicsmethicillin-resistantCCdiseaseddogscatstotal20134caninefelineclinicalsamples2017-2020consistingtypeST18ST5926ST6563multilocustypingt034staphylococcalcassettechromosomeVpredominantlyassociatedIntraclonaldifferentiationt1255t4653non-detectablecompositehybrid+classDNAfingerprintspulsed-fieldgelelectrophoresisessentiallycarriedmediatingmultipleβ-lactamstetracyclines[M]aminoglycosides[]trimethoprimLivestock-associatedincludingELheterogeneouslyfoundlostsubpopulationabsencecorrespondedphenotypessingledetectedintraclonalvariationseemssuccessfulclonecolonizingsmallanimalspet-associatedMethicillin-ResistantClonalComplexMajorLineageDogsCatscatdog

Similar Articles

Cited By