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Feb 17, 2021;9 (2):

Small RNA MTS1338 Confers Pathogenic Properties to Non-Pathogenic .

Oksana Bychenko, Yulia Skvortsova, Rustam Ziganshin, Artem Grigorov, Leonid Aseev, Albina Ostrik, Arseny Kaprelyants, Elena G Salina, Tatyana Azhikina
Author Information
  1. Oksana Bychenko: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
  2. Yulia Skvortsova: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
  3. Rustam Ziganshin: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
  4. Artem Grigorov: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
  5. Leonid Aseev: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
  6. Albina Ostrik: Research Center of Biotechnology, Bach Institute of Biochemistry, 119071 Moscow, Russia.
  7. Arseny Kaprelyants: Research Center of Biotechnology, Bach Institute of Biochemistry, 119071 Moscow, Russia.
  8. Elena G Salina: Research Center of Biotechnology, Bach Institute of Biochemistry, 119071 Moscow, Russia. ORCID
  9. Tatyana Azhikina: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia. ORCID
PMID: 33671144 DOI: 10.3390/microorganisms9020414

Abstract

Small non-coding RNAs play a key role in bacterial adaptation to various stresses. small RNA MTS1338 is upregulated during mycobacteria infection of macrophages, suggesting its involvement in the interaction of the pathogen with the host. In this study, we explored the functional effects of MTS1338 by expressing it in non-pathogenic that lacks the MTS1338 gene. The results indicated that MTS1338 slowed the growth of the recombinant mycobacteria in culture and increased their survival in RAW 264.7 macrophages, where the MTS1338-expressing strain significantly ( < 0.05) reduced the number of mature phagolysosomes and changed the production of cytokines IL-1β, IL-6, IL-10, IL-12, TGF-β, and TNF-α compared to those of the control strain. Proteomic and secretomic profiling of recombinant and control strains revealed differential expression of proteins involved in the synthesis of main cell wall components and in the regulation of iron metabolism (ESX-3 secretion system) and response to hypoxia (). These effects of MTS1338 expression are characteristic for during infection, suggesting that in pathogenic mycobacteria MTS1338 plays the role of a virulence factor supporting the residence of in the host.

Keywords

MTS1338 Mycobacterium smegmatis Mycobacterium tuberculosis macrophages proteomics small non-coding RNA virulence factor

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Grants

  1. 18-15-00332/Russian Science Foundation
Journal Article
Article has an altmetric score of 1

Word Cloud

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