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Toxins
04 03, 2021;13 (4):

Composition and Acute Inflammatory Response from Venom on RAW 264.7 Macrophage Cells.

Suwatjanee Naephrai, Supakit Khacha-Ananda, Pornsiri Pitchakarn, Churdsak Jaikang
Author Information
  1. Suwatjanee Naephrai: Toxicology Section, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  2. Supakit Khacha-Ananda: Toxicology Section, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  3. Pornsiri Pitchakarn: Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ORCID
  4. Churdsak Jaikang: Toxicology Section, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
PMID: 33916734 DOI: 10.3390/toxins13040257

Abstract

(Arboreal Bicoloured Ant) venom induces pain, inflammation, and anaphylaxis in people and has an increased incident in Southeast Asia regions. The bioactive components and mechanism of action of the ant venom are still limited. The aim of this research was to identify the protein composition and inflammatory process of the ant venom by using RAW 264.7 macrophage cells. The major venom proteins are composed of 5' nucleotidase, prolyl endopeptidase-like, aminopeptidase N, trypsin-3, venom protein, and phospholipase A (PLA). The venom showed PLA activity and represented 0.46 μg of PLA bee venom equivalent/μg crude venom protein. The venom induced cytotoxic in a dose- and time-dependent manner with IC approximately at 4.01 µg/mL. The increased levels of COX-2 and PGE were observed after 1 h of treatment correlating with an upregulation of expression. Moreover, the level of expression was obviously increased after 12 h of venom induction. Hence, our results suggested that the induction of pathway could be a direct pathway for the ant venom-induced inflammation.

Keywords

COX-2/mPGES-1 pathway hymenoptera inflammation

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Grants

  1. 083-2562/Faculty of Medicine, Chiang Mai University

MeSH Term

Animals
Ant Venoms
Ants
Cyclooxygenase 2
Dinoprostone
Dose-Response Relationship, Drug
Inflammation
Inflammation Mediators
Insect Proteins
Macrophages
Mice
Prostaglandin-E Synthases
RAW 264.7 Cells
Time Factors
Up-Regulation

Chemicals

Ant Venoms
Inflammation Mediators
Insect Proteins
Ptgs2 protein, mouse
Cyclooxygenase 2
Prostaglandin-E Synthases
Ptges protein, mouse
Dinoprostone
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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