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Neurourology and urodynamics
06, 2021;40 (5): 1126-1132.

Hydrodistention does not alter bladder gene expression profiles in patients with non-Hunner lesion interstitial cystitis/bladder pain syndrome.

Raymond Xu, Jeffrey Schachar, Robert J Evans, Catherine A Matthews, Gopal Badlani, Stephen J Walker
Author Information
  1. Raymond Xu: Department of Urology, Wake Forest Baptist Health, Winston-Salem, North Carolina, USA.
  2. Jeffrey Schachar: Florida Robotic and Minimally Invasive Urogynecology, Plantation, Florida, USA.
  3. Robert J Evans: Department of Urology, Wake Forest Baptist Health, Winston-Salem, North Carolina, USA.
  4. Catherine A Matthews: Department of Urology, Wake Forest Baptist Health, Winston-Salem, North Carolina, USA.
  5. Gopal Badlani: Department of Urology, Wake Forest Baptist Health, Winston-Salem, North Carolina, USA.
  6. Stephen J Walker: Department of Urology, Wake Forest Baptist Health, Winston-Salem, North Carolina, USA. ORCID
PMID: 33942362 DOI: 10.1002/nau.24680

Abstract

AIMS: Gene expression profiling of Bladder biopsies in patients with interstitial cystitis/Bladder pain syndrome (IC/BPS), typically obtained following therapeutic Bladder hydrodistention (HOD), is used to improve our understanding of molecular phenotypes. The objective of this study was to determine if the HOD procedure itself impacts the biopsy gene expression profile and, by extension, whether biopsies from non-HOD bladders are appropriate controls.
METHODS: Bladder biopsies were obtained just before HOD and immediately following HOD from 10 consecutively recruited IC/BPS patients undergoing therapeutic HOD. Biopsies were also obtained from four non-IC/BPS patients who did not undergo HOD (controls). Total RNA was isolated from each of the 24 samples and used to query whole-genome microarrays. Differential gene expression analysis was performed to compare expression profiles of IC/BPS biopsies before and after HOD, and between IC/BPS and control biopsies.
RESULTS: Principal component analysis revealed complete separation between gene expression profiles from IC/BPS and control samples (q ≤ 0.05) and while IC/BPS samples before and after HOD showed no significant differences in expressed genes, 68 transcripts were found to be significantly different between IC/BPS and control samples (q ≤ 0.05).
CONCLUSIONS: The Bladder HOD procedure itself does not significantly change gene expression within the IC/BPS patient Bladder, a finding that provides evidence to support the use of biopsies from non-IC/BPS patients that have not undergone HOD as controls for gene expression studies.

Keywords

bladder biopsy hydrodistention interstitial cystitis/bladder pain syndrome molecular profiling

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Grants

  1. R01 DK124599/NIDDK NIH HHS
  2. R21 DK106554/NIDDK NIH HHS

MeSH Term

Biopsy
Cystitis, Interstitial
Humans
Pain
Transcriptome
Journal Article Research Support, N.I.H., Extramural
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0HODexpressionIC/BPSbladderbiopsiesgenepatientssamplesinterstitialcystitis/bladderpainsyndromeobtainedcontrolsprofilescontrolprofilingfollowingtherapeutichydrodistentionusedmolecularprocedurebiopsynon-IC/BPSq ≤ 005significantlyAIMS:Genetypicallyimproveunderstandingphenotypesobjectivestudydetermineimpactsprofileextensionwhethernon-HODbladdersappropriateMETHODS:Bladderjustimmediately10consecutivelyrecruitedundergoingBiopsiesalsofourundergoTotalRNAisolated24querywhole-genomemicroarraysDifferentialanalysisperformedcompareRESULTS:Principalcomponentanalysis revealedcompleteseparationshowedsignificantdifferencesexpressedgenes68transcriptsfounddifferentCONCLUSIONS:changewithinpatientfindingprovidesevidencesupportuseundergonestudiesHydrodistentionalternon-Hunnerlesion

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