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Probiotics and antimicrobial proteins
12, 2021;13 (6): 1798-1807.

β-Defensin from the Asian Sea Bass, Lates calcarifer: Molecular Prediction and Phylogenetic Analysis.

Athira Raveendran, Dhanya Lenin K L, Anju M V, Neelima S, Anooja V V, Athira P P, Archana K, Rosamma Philip, Swapna P Antony
Author Information
  1. Athira Raveendran: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  2. Dhanya Lenin K L: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  3. Anju M V: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  4. Neelima S: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  5. Anooja V V: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  6. Athira P P: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  7. Archana K: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  8. Rosamma Philip: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India.
  9. Swapna P Antony: Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi-16, 682 016, Kerala, India. swapnapantony@gmail.com. ORCID
PMID: 34043156 DOI: 10.1007/s12602-021-09804-5

Abstract

Antimicrobial peptides (AMPs) are an important element of the innate immune system of all living organisms and serve as a barrier that safeguards the organisms against a wide range of pathogens. Fishes are proven to be a prospective source of AMPs, and β-defensins form an important family of AMPs with potent antimicrobial, chemotactic and immunomodulatory activities. The present study reports a β-defensin AMP sequence (Lc-BD) from the Asian sea bass, Lates calcarifer, a commercially important fish species in tropical and subtropical regions of Asia and the Pacific. A 202-bp cDNA fragment with an open reading frame encoding 63 amino acids (aa) was obtained from the mRNA of gill tissue by RT-PCR. The deduced aa sequence of Lc-BD possessed a signal and a mature peptide region with 20 and 43 aa residues, respectively. Lc-BD was characterized at the molecular level, and a molecular weight of 5.24 kDa and a net charge of +4.5 was predicted for the mature peptide. The molecular characterization of Lc-BD revealed the presence of three intramolecular disulphide bonds involving the six conserved cysteine residues in the sequence, and the phylogenetic analysis of Lc-BD showed a close relationship with β-defensins from fishes like Siniperca chuatsi, Argyrosomus regius, Trachinotus ovatus and Oplegnathus fasciatus.

Keywords

Antimicrobial peptide Molecular characterization Phylogeny Sea bass β-defensin

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MeSH Term

Amino Acids
Animals
Perciformes
Phylogeny
Prospective Studies
beta-Defensins

Chemicals

Amino Acids
beta-Defensins
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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