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Nutrients
May 28, 2021;13 (6):

Dietary Intake of -3 PUFA-Enriched Hen Eggs Changes Inflammatory Markers' Concentration and Treg/Th17 Cells Distribution in Blood of Young Healthy Adults-A Randomised Study.

Nikolina Kolobarić, Ines Drenjančević, Anita Matić, Petar Šušnjara, Zrinka Mihaljević, Martina Mihalj
Author Information
  1. Nikolina Kolobarić: Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, Croatia. ORCID
  2. Ines Drenjančević: Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, Croatia. ORCID
  3. Anita Matić: Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, Croatia.
  4. Petar Šušnjara: Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, Croatia.
  5. Zrinka Mihaljević: Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, Croatia. ORCID
  6. Martina Mihalj: Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, Croatia. ORCID
PMID: 34071714 DOI: 10.3390/nu13061851

Abstract

In the present study, we aimed to determine the effects of -3 polyunsaturated acid (PUFA) supplementation (~1053 mg/per day), i.e., α-linolenic (~230 mg), eicosapentaenoic (~15 mg), and docosahexaenoic acid (~105 mg), through hen eggs, on pro- and anti-inflammatory parameters in healthy individuals (23.8 ± 2.57 years old). Here, we demonstrate differential effects of regular hen eggs ( = 21; W/M = 10/11) and -3 PUFA-enriched hen eggs ( = 19; W/M = 10/9) consumption on the serum levels of lipid mediators, representation of peripheral T helper cell subsets (recently activated T-helper cells, nTreg, Th17 and non-Th17-IL-17A secreting T-helper lymphocytes) and their functional capacity for cytokine secretion. Both diets significantly altered systemic levels of pro-inflammatory and inflammation resolving lipid mediators; however, only the -3 PUFAs group showed a significant shift towards anti-inflammatory prostanoids and increased levels of pro-resolving oxylipins. Both study groups showed reduced frequencies of peripheral nTreg lymphocytes and decreased rates of peripheral Th17 cells. Their functional capacity for cytokine secretion was significantly altered only in the -3 PUFAs group in terms of increased transforming growth factor β-1 and reduced interleukin 6 secretion. Diet supplemented with -3 PUFAs alters immune response towards inflammation resolving conditions through effects on lipid mediators and cytokine secretion by T lymphocytes in human model without underlying comorbidities.

Keywords

T lymphocytes dietary supplements eicosanoids fatty acids inflammation

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Grants

  1. # KK.01.1.1.01.0010/European Structural and Investment Funds, to Science Centre of Excellence for Personalized Health Care, Josip Juraj Strossmayer University of Osijek, Scientific Unit for Research, Production and Medical Testing of Functional Food

MeSH Term

Adult
Animals
Chickens
Diet
Double-Blind Method
Eggs
Fatty Acids, Omega-3
Female
Humans
Inflammation
Male
T-Lymphocytes, Regulatory
Th17 Cells
Young Adult

Chemicals

Fatty Acids, Omega-3
Journal Article Randomized Controlled Trial

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