De novo transcriptome sequencing assisted identification of terpene synthases from black pepper () berry.
Johnson K George, Sreekumar Shelvy, Abdulkabeer Muhammed Fayad, Palaniyandi Umadevi, U B Angadi, Mir Asif Iquebal, Sarika Jaiswal, Anil Rai, Dinesh Kumar
Author Information
Johnson K George: ICAR - Indian Institute of Spices Research, Kozhikode, Kerala India.
Sreekumar Shelvy: ICAR - Indian Institute of Spices Research, Kozhikode, Kerala India.
Abdulkabeer Muhammed Fayad: ICAR - Indian Institute of Spices Research, Kozhikode, Kerala India.
Palaniyandi Umadevi: ICAR - Indian Institute of Spices Research, Kozhikode, Kerala India. ORCID
U B Angadi: ICAR - Indian Agricultural Statistics Research Institute, New Delhi, India.
Mir Asif Iquebal: ICAR - Indian Agricultural Statistics Research Institute, New Delhi, India.
Sarika Jaiswal: ICAR - Indian Agricultural Statistics Research Institute, New Delhi, India.
Anil Rai: ICAR - Indian Agricultural Statistics Research Institute, New Delhi, India.
Dinesh Kumar: ICAR - Indian Agricultural Statistics Research Institute, New Delhi, India.
Though the volatile profiles of black pepper have been reported already, the information on terpene synthase family genes is not known. In this study, using a combinatorial approach, the berry hybrid transcriptome assembly of llumina and nanopore sequencing, the entire terpene synthase family responsible for the biosynthesis of the flavor-imparting volatiles in black pepper berries was profiled. The profile shows 98 terpene synthases from various terpene synthesis pathways. Three important monoterpene synthases were also validated by targeted amplification, sequencing and homology modeling. This study provides the first of its kind information on the terpene synthase family profile in which is potentially a major step for further characterization of the functional terpene synthase genes in black pepper. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-021-00986-4.
